4-Nitrophenyl Phosphate (sodium salt hydrate) (Synonyms: p-Nitrophenyl Phosphate, para-Nitrophenyl Phosphate, 4-NPP, pNPP) |
| Catalog No.GC49158 |
4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases.
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Cas No.: 333338-18-4
Sample solution is provided at 25 µL, 10mM.
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4-Nitrophenyl phosphate (pNPP) is a chromogenic substrate for alkaline phosphatase.1 Upon hydrolysis by alkaline phosphatase, 4-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of alkaline phosphatase activity. It has been commonly used in ELISAs.
1.Lorenz, U.Protein tyrosine phosphatase assaysCurr. Protoc. Immunol.9311.17.11-11.17.12(2011)
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