5-Methyl-CTP |
| Catalog No.GB20105 |
5-Methyl-CTP is a modified nucleoside triphosphate, commonly used to substitute the naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
5-Methyl-CTP is a modified nucleoside triphosphate, commonly used to substitute the naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis. Methylation of the cytosine C-5 position is a common, post-transcriptional modification for mRNA. Incorporation of chemically modified 5-methyl-CTP can increase mRNA stability and protein expression, and meanwhile, reduce interaction of innate immune receptors with in vitro-transcribed (IVT) mRNA. Apart from its application in encoding antigens for the activation of immune response against a variety of diseases such as COVID-19, IVT mRNA can also express the nanobody/variable domain of heavy chain of heavy-chain antibody targeting the intracellular components, enabling intracellular antigen labeling and manipulation. References: 1. Rahman MM, Zhou N, Huang J. An overview on the development of mRNA-based vaccines and their formulation strategies for improved antigen expression in vivo. Vaccines (Basel), 2021, 9(3): 244. 2. Lu J, Lu G, Tan S, et al. A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice. Cell Research, 2020, 30(10): 936-939. 3. Zhou X, Hao R, Chen C, et al. Rapid delivery of nanobodies/VHHs into living cells via expressing in vitro-transcribed mRNA. Molecular Therapy - Methods & Clinical Development, 2020, 17: 401-408.
| Cas No. | SDF | ||
| Formula | C10H18N3O14P3 (free acid) | M.Wt | 497.1 (free acid) |
| Solubility | Storage | Store at -20°C or below | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Quality Control & SDS
- View current batch:
-
Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *