740 Y-P (Synonyms: 740YPDGFR; PDGFR 740Y-P) |
| Catalog No.GC16157 |
740 Y-P is an activator of PI3K with concentration of 20 μM [1].
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1236188-16-1
Sample solution is provided at 25 µL, 10mM.
740 Y-P is an activator of PI3K with concentration of 20 μM [1].
PI3K (phosphoinositide 3-kinases, PI3Ks) is an enzyme and plays an important role in the fundamental cellular processes, such as vesicular trafficking, cell degranulation, cell migration, and glucose transporter. It has been reported that over-expression of PI3K was correlated with a variety kind of cancers [2].
740 Y-P is a potent PI3K activator and plays an important role in PI3K/AKT signaling pathway. When tested with human melanoma MNT-1 cells, 20 μM 740 Y-P for 24 hours treatment significantly reduced the number of M6PR-positive vacuoles induced by sucrose via activating PI3K [1]. In cerebellar granule cells in the circumstance of serum deprivation, 740 Y-P treatments reduced the cell death rate via binding to p85 which was correlated with PI 3-kinase-dependent phosphorylation of Akt process [3].
References:
[1]. Bin, B.H., et al., Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One, 2014. 9(8): p. e105965.
[2]. Kong, B., et al., A subset of metastatic pancreatic ductal adenocarcinomas depends quantitatively on oncogenic Kras/Mek/Erk-induced hyperactive mTOR signalling. Gut, 2015.
[3]. Williams, E.J. and P. Doherty, Evidence for and against a pivotal role of PI 3-kinase in a neuronal cell survival pathway. Mol Cell Neurosci, 1999. 13(4): p. 272-80.
| Cell experiment [1]: | |
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Cell lines |
Human melanoma MNT-1 cells |
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Preparation method |
Limited solubility. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reaction Conditions |
24 h |
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Applications |
740 Y-P induces the generation of M6PR-positive puncta which is similar to the sucrose-induced vacuoles. 740 Y-P also prominently reduces the number of M6PR-positive vacuoles triggers by the sucrose treatment. |
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References: 1. Bin BH, Bhin J, Yang SH et al. Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One. 2014 Aug 29;9(8):e105965. |
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| Cas No. | 1236188-16-1 | SDF | |
| Synonyms | 740YPDGFR; PDGFR 740Y-P | ||
| Formula | C141H222N43O39PS3 | M.Wt | 3270.72 |
| Solubility | ≥ 163.536mg/mL in DMSO | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.3057 mL | 1.5287 mL | 3.0574 mL |
| 5 mM | 0.0611 mL | 0.3057 mL | 0.6115 mL |
| 10 mM | 0.0306 mL | 0.1529 mL | 0.3057 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Fu Fang Gang Liu liquid (FFGL) inhibits the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. D Cells were treated with FFGL (80 μg/mL) with various concentrations of 740 Y-P for 48 h. Cell viability was measured using the cell-counting kit 8 assay.
Similarly, we treated HeLa cells with the PI3K activator 740 Y-P (GlpBio, Montclair,CA, USA) (0, 12.5, 25, 50, 100, 200 μg/mL) in combination with FFGL (80 μg/mL) for 48 h.
Bmc Complement Med 23.1 (2023): 128. PMID: 37081536 IF: 2.838 -
Related Biological Data
THC inhibits the activation of the PI3K/AKT/mTOR pathway. g-i. Next, the same cells were treated for 48 hours to 15 μM 740Y-P (PI3K activator) alone, 100 μM THC alone, or 15 μM 740Y-P plus 100 μM THC.
We ordered the 740Y-P from GlpBio Technology (Montclair, CA, USA).
Arch Clin Psychiat 50.6 (2023). IF: 0.9839
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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