8-iso Prostaglandin E2-d4 (Synonyms: 8epi PGE2d4, 8iso PGE2d4)

8-iso Prostaglandin E₂-d₄ (8-iso PGE₂-d₄) contains four deuterium atoms at the hydroxyethyl 3, 3',4, and 4' positions. It is intended for use as an internal standard for the quantification of 8-iso PGE₂ by GC- or LC-mass spectrometry. 8-iso PGE₂ is one of several isoprostanes produced from arachidonic acid during lipid peroxidation.¹ It is a potent renal vasoconstrictor in the rat.^1,2 8-iso PGE₂ inhibits U-46619 or I-BOP-induced platelet aggregation with IC₅₀ values of 0.5 and 5 µM, respectively.³ When infused into the renal artery of the rat at a concentration of 4 mg/kg/min, 8-iso PGE₂ decreases the GFR and renal plasma flow by 80% without affecting blood pressure.¹

1.Morrow, J.D., Minton, T.A., Mukundan, C.R., et al.Free radical-induced generation of isoprostanes in vivo. Evidence for the formation of D-ring and E-ring isoprostanesThe Journal of Biological Chemisty2694317-4326(1994) 2.Hoffman, S.W., Moore, S., and Ellis, E.F.Isoprostanes: Free radical-generated prostaglandins with constrictor effects on cerebral arteriolesStroke28844-884(1997) 3.Longmire, A.W., Roberts, L.J., and Morrow, J.D.Actions of the E2-isoprostane, 8-iso-PGE2, on the platelet thromboxane/endoperoxide receptor in humans and rats: Additional evidence for the existence of a unique isoprostane receptorProstaglandins48247-256(1994)