8-O-Acetylharpagide

8-O-Acetylharpagide is an iridoid compound isolated from Ajuga reptile. The compound has antibacterial, antispasmodic, cardiotonic and antipyretic properties^{[1] [2]}.

8-O-Acetylharpagide (1-10μM; 24h) treatment inhibited ROS in a concentration-dependent manner in old HDFs. Low concentrations (less than 10μM) of 8-O-Acetylharpagide (5-50μg/ml; 48h) can fully inhibit the senescence-associated phenotypic expression of p53 and SA-β-gal in aged HDFs^[1]. Different concentrations of 8-O-Acetylharpagide (100-250μg/ml; 24h) were active against Bacillus anthracis, but inactive against Staphylococcus aureus, Streptococcus agalactiae, Staphylococcus epidermidis, Aeromonas sobria, Shigella boydii, Vibrio cholerae, Corynebacterium diphtheriae, Salmonella typhi (Gram-positive bacteria), and Pseudomonas aeruginosa (Gram-positive bacteria) ^[2].

8-O-Acetylharpagide (100-1000µg/ml; immersion 20s) showed spasmolytic effects on isolated ileum and cardiotonic effects on isolated rabbit hearts at a test dose of 100µg/ml. No harmful or toxic effects of 8-O-Acetylharpagide (1-100 μg; po; 1 time) were detected^[2].

References:
[1].Shafi N, Khan G A, Arfan M, et al. Isolation and pharmacological screening of 8-O-acetyl harpagide from Ajuga bracteosa Wall[J]. Biological Sciences-PJSIR, 2004, 47(3): 176-179.
[2].Hsu W H, Lin B Z, Leu J D, et al. Involvement of 8-O-acetylharpagide for Ajuga taiwanensis mediated suppression of senescent phenotypes in human dermal fibroblasts[J]. Scientific reports, 2020, 10(1): 19731.