Alamethicin (Synonyms: Antibiotic U-22324) |
| Catalog No.GC11255 |
antibiotic peptide
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 27061-78-5
Sample solution is provided at 25 µL, 10mM.
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Alamethicin is an antibiotic peptide.
Plenty of peptide antibiotics have been identified in the past half-century, falling into two classes, non-ribosomally synthesized peptides, such as polymyxins, gramicidins, bacitracins, glycopeptides, and ribosomally synthesized (natural) peptides. The former are usually modified and produced by bacteria, while the latter are produced by all species as a major component of the host defense molecules.
In vitro: Alamethicin was identified as an antibiotic peptide belonging to membrane active peptides of fungal origin with an unusual amphiphilic amino acid, 2-aminoisobutyric acid, which can induce helical peptide structures strongly, leading to the formation of voltage-gated ion channels in the cell membrane bilayers. Alamethicin is also widely used as agent to induce various defense responses and physiological in eukaryotic cells [1]. In addition, alamethicin was quite often used to evaluate voltage gating, ion channel assembly, as well as peptide-membrane interactions [2, 3].
In vivo: Up to now, alamethicin is only for in-vitro usage and there is no animal in-vivo study reported.
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Maischak, H. ,Zimmermann, M.R.,Felle, H.H., et al. Alamethicin-induced electrical long distance signaling in plants. Plant Signal.Behav. 5(8), 988-990 (2010).
[2] Jones, L. R.,Maddock, S.W. and Besch, H.R., Jr. Unmasking effect of alamethicin on the (Na+,K+)-ATPase, β-adrenergic receptor-coupled adenylate cyclase, and cAMP-dependent protein kinase activities of cardiac sarcolemmal vesicles. The Journal of Biological Chemisty 255(20), 9971-9980 (1980).
[3] Pan, J. ,Tristram-Nagle, S. and Nagle, J.F. Alamethicin aggregation in lipid membranes. Journal of Membrane Biology 231(1), 1-36 (2009).
| Cas No. | 27061-78-5 | SDF | |
| Synonyms | Antibiotic U-22324 | ||
| Chemical Name | (3S,12R)-1-((S)-1-((6S,12S,15S,21S,30S)-1-((R)-1-(2-acetamido-2-methylpropanoyl)pyrrolidin-2-yl)-15-(3-amino-3-oxopropyl)-30-isobutyl-21-isopropyl-3,3,6,9,9,12,18,18,24,24,33,33-dodecamethyl-1,4,7,10,13,16,19,22,25,28,31-undecaoxo-2,5,8,11,14,17,20,23,26, | ||
| Canonical SMILES | CC(C[C@H](NC(CNC(C(C)(NC([C@@H](NC(C(C)(NC([C@@H](NC([C@@H](NC(C(C)(NC([C@@H](NC(C(C)(NC([C@H]1CCCN1C(C(C)(NC(C)=O)C)=O)=O)C)=O)C)=O)C)=O)C)=O)CCC(N)=O)=O)C)=O)C(C)C)=O)C)=O)=O)C(NC(C)(C(N2CCC[C@H]2C(N[C@H](C(NC(C)(C(NC(C)(C(N[C@@H](C(N[C@@H](C(N[C@H | ||
| Formula | C92H150N22O25 | M.Wt | 1964.3 |
| Solubility | ≤10mg/ml in methanol;10mg/ml in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.5091 mL | 2.5454 mL | 5.0909 mL |
| 5 mM | 0.1018 mL | 0.5091 mL | 1.0182 mL |
| 10 mM | 0.0509 mL | 0.2545 mL | 0.5091 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 6 reference(s) in Google Scholar.)
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