13C20,15N10-Cyclic di-GMP (sodium salt) (Synonyms: 13C20,15N10-c-di-GMP, 13C20,15N10-Cyclic diguanylate, 13C20,15N10-3’,5’-Cyclic diguanylic Acid) |
| رقم الكتالوجGC49912 |
An internal standard for the quantification of cyclic di-GMP
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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13C20,15N10-Cyclic di-GMP is intended for use as an internal standard for the quantification of cyclic di-GMP by GC- or LC-MS. Cyclic di-GMP is a second messenger in bacteria involved in diverse prokaryotic processes, including biofilm formation, motility, virulence, and cell cycling.1,2 In eukaryotic cells, cyclic di-GMP is detected by and binds to the transmembrane protein stimulator of interferon genes (STING; Kd = 1.21 µM), leading to activation of the innate immune system.3,4 It has been used at a preset molar ratio with STING dimers in binding assays to determine the binding constants of particularly tight binding partners, such as 2’3’-cGAMP. Cyclic di-GMP induces IFN-β mRNA expression in vitro (EC50 = 537.8 nM) but less potently than 2’3’-cGAMP , 3’2’-cGAMP, 3’3’-cGAMP , and 2’2’-cGAMP .
1.RÖmling, U., Galperin, M.Y., and Gomelsky, M.Cyclic di-GMP: The first 25 years of a universal bacterial second messengerMicrobiol. Mol. Biol. Rev.77(1)1-52(2013) 2.MartÍnez, L.C., and Vadyvaloo, V.Mechanisms of post-transcriptional gene regulation in bacterial biofilmsFront. Cell. Neurosci.4(38)1-15(2014) 3.Schaap, P.Cyclic di-nucleotide signaling enters the eukaryote domainIUBMB Life65(11)897-903(2013) 4.Zhang, X., Shi, H., Wu, J., et al.Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STINGMol. Cell51(2)226-235(2015)
| Cas No. | SDF | ||
| المرادفات | 13C20,15N10-c-di-GMP, 13C20,15N10-Cyclic diguanylate, 13C20,15N10-3’,5’-Cyclic diguanylic Acid | ||
| Canonical SMILES | O=[13C]1[15NH][13C]([15NH2])=[15N][13C]2=[13C]1[15N]=[13CH][15N]2[13C@@H]([13C@@H]3O)O[13C@@]([H])([13C@]3(OP([O-])(O[13CH2][13C@]([H])([13C@]4(O5)[H])O[13C@H]([13C@@H]4O)[15N]6[13C]([15N]=[13C]([15NH]7)[15NH2])=[13C]([15N]=[13CH]6)[13C]7=O)=O)[H])[13CH2]OP5([O-])=O.[Na+].[Na+] | ||
| Formula | [13C]20H22[15N]10O14P2 • 2Na | M.Wt | 764.2 |
| الذوبان | Water: soluble | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3086 mL | 6.5428 mL | 13.0856 mL |
| 5 mM | 0.2617 mL | 1.3086 mL | 2.6171 mL |
| 10 mM | 0.1309 mL | 0.6543 mL | 1.3086 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)
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