5-Ethynyl-2'-deoxyuridine (Synonyms: 5-EdU, EdU, 2'-deoxy-5-Ethynyluridine) |
رقم الكتالوجGC42504 |
يعتبر 5-إثيلنيل-2'-ديوكسيوريدين (EdU)، كما يُعرف باسم مشابه للثايميدين، والذي يحتوى على مجموعة الكايلد المستبدلة بالألكاين في الموضع 5 من حلقة البيراميدين، قادرًا على أن يتضمَّن بسهولة داخل الحامض النووى خلال التخصُّص[1].
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Cas No.: 61135-33-9
Sample solution is provided at 25 µL, 10mM.
5-ethynyl-2′-deoxyuridine (EdU), as a thymidine analoghas, a terminal alkyne group replacing a methyl group at the 5 position of the pyrimidine ring and can be readily incorporated into DNA during synthesis[1]. EdU is a inhibitor of the cell growth of human breast cancer cells (MCF-7 and MDA-MP-231) with the IC50 of 0.4 μM for MCF-7 cells and 4.4 μM for MDA-MB-231 cells[2].
In vitro, 10 μMEdU induced gamma-H2AX foci for 51D1 and KO40 in CHO cells for DNA damage responses[3]. In vitro experiment it shown that A549 cells were treated with 20 μM EdU for 6 h increased the level of expression of γH2AX and ATM-S1981P by 88% and 116%, respectively[4].
In vivo efficacy test it shown that femalJKe mice were administrated 10, 20, 50, 100 or 200 mg/kg EdU intraperitoneally, there is an increase in the number of EdU positive cells in the DG slightlyin a dose-dependent manner[5]. In vivo, few EdU-positive cells were observed in Wistar and GK rats at a dose of 5 mg/kg and 25 mg/kg EdU intraperitoneally. Prominent EdU-positive cells were observed in Wistar and GK rats at a dose of 100 mg/kg and 200 mg/kg, respectively[6]. In vivo, use of 5-Ethynyl-2'-deoxyuridine (EdU) incorporation to in vivo monitor T lymphocyte proliferation by flow cytometry with an adoptive transfer model. The result shown that the percentage of EdU-positive cells increased in a dose-dependent manner, and the saturated dose of EdU was 20mg/kg. Intraperitoneal and intravenous injection had no differences in lymphocyte proliferation detection with EdU in vivo[7].
References:
[1] Kaiser CL, et al. 5-Ethynyl-2'-deoxyuridine labeling detects proliferating cells in the regenerating avian cochlea. Laryngoscope. 2009 Sep;119(9):1770-5.
[2] Meneni S, et al. (2007) 5-Alkynyl-2′-deoxyuridines: chromatography-free synthesis and cytotoxicity evaluation against human breast cancer cells. Bioorg Med Chem 15: 3082–3088.
[3] Haskins JS, et al. Evaluating the Genotoxic and Cytotoxic Effects of Thymidine Analogs, 5-Ethynyl-2'-Deoxyuridine and 5-Bromo-2'-Deoxyurdine to Mammalian Cells. Int J Mol Sci. 2020 Sep 10;21(18):6631.
[4] Zhao H, et al. DNA damage signaling, impairment of cell cycle progression, and apoptosis triggered by 5-ethynyl-2'-deoxyuridine incorporated into DNA. Cytometry A. 2013 Nov;83(11):979-88.
[5] Zeng C, et al. Evaluation of 5-ethynyl-2'-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.
[6] Guo J, Li D, et al. Detecting DNA synthesis of neointimal formation after catheter balloon injury in GK and in Wistar rats: using 5-ethynyl-2'-deoxyuridine. Cardiovasc Diabetol. 2012 Dec 13;11:150.
[7] Sun X, Zhang C, et al. Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation. Int Immunopharmacol. 2016 Dec;41:56-65.
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