Baohuoside I (Synonyms: Icariin II, Icariside II) |
| رقم الكتالوجGN10507 |
Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 113558-15-9
Sample solution is provided at 25 µL, 10mM.
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Baohuoside I, a flavonoid isolated from Epimedium koreanum Nakai, acts as an inhibitor of CXCR4, downregulates CXCR4 expression, induces apoptosis and shows anti-tumor activity [1].
Baohuoside I exhibited strong inhibition against the α-glucosidase while icariin and epimedin A, B and C were weak or inactive. All compounds were inactive against pancreatic α-amylase. The IC50 of baohuoside I was 28.9 μmol/L[2].A549 cells were evidently inhibited by Baohuoside I in a time- and concentration-dependent manner. IC50 was approximately 25.1 μM at 24 h, 11.5μM at 48 h and 9.6μM at 72 h, respectively [3].
Baohuoside I prolonged graft survival on rat. A negative control group showed a mean survival time of 6.5±0.5 days. Monotherapy with a subtherapeutic oral dose of Baohuoside I (8.0 mg/kg/day) or FK506 (1.0 mg/kg/day) significantly prolonged cardiac allograft survival up to 9.0±2.0 days or 16.4±6.3 days, compared with negative controls. Results with combination therapy of these two drugs in the same doses (25.0±2.0 days) indicate that an additive prolongation of graft survival was produced when compared with monotherapy with Baohuoside I or FK506[4].
References:
[1]. Kim B, et al. Baohuoside I suppresses invasion of cervical and breast cancer cells through the downregulation of CXCR4 chemokine receptor expression. Biochemistry. 2014 Dec 9;53(48):7562-9.
[2]. M.A.T. Phan, J. Wang, J.Y. Tang, et al. Evaluation of α-glucosidase inhibition potential of some flavonoids from Epimedium brevicornum L. WT-Food Science and Technology, 53 (2013), pp. 492-498.
[3]. Song J, et al. Reactive oxygen species-mediated mitochondrial pathway is involved in Baohuoside I-induced apoptosis in human non-small cell lung cancer. Chem Biol Interact. 2012 Jul 30;199(1):9-17.
[4].A. Ma, S. Qi, D. Xu, X. Zhang, P. Daloze, H. Chen . Baohuoside-1, a novel immunosuppressive molecule, inhibits lymphocyte activation in vitro and in vivo. Transplantation, 78 (2004), pp. 831-838.
| Cell experiment [1]: | |
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Cell lines |
Human cervical cancer HeLa cells |
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Preparation Method |
Baohuoside I was dissolved in dimethyl sulfoxide (DMSO) as a 10 mM stock solution and stored at 4℃. |
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Reaction Conditions |
Invasion assay by polycarbonate membranes with a pore size of 8 μm，25 μM baohuoside I |
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Applications |
Baohuoside I correlates with cervical cancer cell downregulation of CXC chemokine receptor 4. CXCL12 induced the invasion of cervical cancer cells, and baohuoside I effectively abrogated it. |
| Animal experiment [2]: | |
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Animal models |
Inbred female C57/BL/6J mice |
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Preparation Method |
Baohuoside I is stored in pure ethanol and suspended in 0.9% sterile saline intraperitoneal injection. |
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Dosage form |
Intraperitoneal injection, 20 mg/kg/d |
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Applications |
Mice received Baohuoside I by daily i.p. injection from day 0 to day 4. They were immunized with sheep red blood cells (SRBC) at day 0 and bled at day 5. The results showed significant suppression of anti-SRBC antibody responses at dosage schedules of 18.8 mg/ kg/day and above (P<0.001). |
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References: [1]. Kim B, et al. Baohuoside I suppresses invasion of cervical and breast cancer cells through the downregulation of CXCR4 chemokine receptor expression. Biochemistry. 2014 Dec 9;53(48):7562-9. [2]. Li SY, Ping G, Geng L, et al. Immunopharmacology and toxicology of the plant flavonoid baohuoside-1 in mice. Int J Immunopharmacol 1994; 16: 227. |
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| Cas No. | 113558-15-9 | SDF | |
| المرادفات | Icariin II, Icariside II | ||
| Chemical Name | 5,7-dihydroxy-2-(4-methoxyphenyl)-8-(3-methylbut-2-enyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxychromen-4-one | ||
| Canonical SMILES | CC1C(C(C(C(O1)OC2=C(OC3=C(C2=O)C(=CC(=C3CC=C(C)C)O)O)C4=CC=C(C=C4)OC)O)O)O | ||
| Formula | C27H30O10 | M.Wt | 514.18 |
| الذوبان | DMF: 10 mg/ml,DMSO: 10 mg/ml,Ethanol: 0.5 mg/ml,PBS (pH 7.2): 0.1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9448 mL | 9.7242 mL | 19.4484 mL |
| 5 mM | 0.389 mL | 1.9448 mL | 3.8897 mL |
| 10 mM | 0.1945 mL | 0.9724 mL | 1.9448 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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