Cytotoxicity LDH Assay Kit: Principle, Applications, and Operation Guide

In life science research, the assessment of cytotoxicity is an important indicator for measuring the impact of compounds or treatments on cell health. To accurately and quickly detect the extent of cell damage, Glpbio offers a cytotoxicity LDH assay kit. This kit measures cell damage by quantifying the activity of lactate dehydrogenase (LDH) released into the culture medium, providing researchers with an efficient and reliable detection tool.

Principle of the Cytotoxicity LDH Assay Kit

LDH is an enzyme present in the cytoplasm that plays a key role in cellular energy metabolism. When the cell membrane is damaged, LDH is released into the culture medium. Since the released LDH is relatively stable in vitro, measuring the amount of LDH in the culture medium can serve as a reliable indicator of the number of dead and damaged cells.

The working principle of the cytotoxicity LDH assay kit is based on the enzymatic activity of LDH. Specifically, LDH can catalyze the conversion of lactate to pyruvate and NADH, with NADH subsequently reducing a water-soluble tetrazolium salt (yellow) to formazan (red) through an electron carrier. In this reaction, the absorbance of formazan is proportional to the concentration of LDH. Thus, by measuring changes in absorbance, the activity of LDH can be calculated accurately, allowing the inference of the number of dead and damaged cells.

Applications of the Cytotoxicity LDH Assay Kit

The cytotoxicity LDH assay kit has extensive application value in life science research. Here are some major application scenarios:

Drug screening and toxicity assessment

In drug development, evaluating the toxicity of candidate drugs on cells is an essential step. The cytotoxicity LDH assay kit can quickly and accurately detects the extent of cell damage after drug treatment, providing strong support for drug screening and toxicity assessment.

Apoptosis and necrosis research

Apoptosis and necrosis are two main forms of cell death. By detecting the amount of LDH release, these two types of cell death can be distinguished, enabling further research on their molecular mechanisms and regulatory pathways.

Disease model research

In disease model research, the cytotoxicity LDH assay kit can be used to assess the extent of cell damage under disease conditions, thereby revealing the molecular mechanisms of disease occurrence and development and providing new targets for disease treatment.

Biomaterial safety evaluation

For novel biomaterials, such as nanomaterials and biomedical materials, safety evaluation is crucial. The cytotoxicity LDH assay kit can be used to detect the potential toxicity of these materials to cells, providing scientific evidence for the safe application of biomaterials.

Operating Instructions for the Cytotoxicity LDH Assay Kit

Glpbio's cytotoxicity LDH assay kit is designed to be simple and easy to operate. Here are the specific steps:

Reagent preparation

Ensure that all reagents are prepared and preheated according to the instructions. Pay special attention to fully dissolving and mixing the reagents before use.

Sample processing

For adherent cells, first remove the culture medium, wash the cells with PBS, then add an appropriate volume of lysis solution from the kit, lyse the cells, and collect the lysate. For suspended cells, directly centrifuge to collect and lyse the cells.

LDH activity measurement

Mix the lysate with the reaction solution from the kit, and incubate for the required time and temperature according to the instructions. After incubation, measure the absorbance of the reaction solution.

Data calculation and analysis

Based on the absorbance values, use the standard curve or calculation formula provided in the kit to calculate the LDH activity units. Further, infer the number of dead and damaged cells based on the LDH activity units.

Advantages of Glpbio's Cytotoxicity LDH Assay Kit

As a global leader in high-performance life science products, Glpbio's cytotoxicity LDH assay kit has the following prominent advantages:

High sensitivity and accuracy

The kit employs advanced enzymatic reaction principles to ensure high sensitivity and accuracy, accurately reflecting the extent of cell damage.

Simple and quick operation

The kit provides detailed operation instructions, with concise and clear steps, requiring no complicated sample pre-treatment, greatly saving experimental time.

Wide applicability

The kit is suitable for various cell types, including adherent and suspended cells, meeting different experimental needs.

High quality and stability

Glpbio adheres to the core philosophy of "ensuring the quality of every product," with reagents in the kit having excellent purity and stability, ensuring the reliability and reproducibility of experimental results.

The cytotoxicity LDH assay kit is an indispensable tool in life science research. With its outstanding product performance and wide application value, Glpbio provides strong support to scientists worldwide. Whether in drug screening, disease model research, or biomaterial safety evaluation, the cytotoxicity LDH assay kit plays a crucial role. Choose Glpbio for high-quality life science reagents to make your research journey smoother!