C24:1 Ceramide (d18:1/24:1(15Z)) (Synonyms: Ceramide (d18:1/24:1(15Z)); Cer(d18:1/24:1(15Z)); Nervonic Ceramide; N-Nervonoyl-D-erythro-Sphingosine) |
| رقم الكتالوجGC43078 |
C24:1 Ceramide (d18:1/24:1(15Z)) is an abundant naturally occurring ceramide that regulates many different biological activities, such as apoptosis, cell differentiation, smooth muscle cell proliferation, and inhibition of the mitochondrial respiratory chain.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 54164-50-0
Sample solution is provided at 25 µL, 10mM.
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C24:1 Ceramide (d18:1/24:1(15Z)) is an abundant naturally occurring ceramide that regulates many different biological activities, such as apoptosis, cell differentiation, smooth muscle cell proliferation, and inhibition of the mitochondrial respiratory chain[1, 2]. C24:1 Ceramide is abundantly expressed in brain tissue and accumulates in patients with Gaucher disease[3]. C24:1 Ceramide can be used as a standard hexosylceramide for hydrophilic interaction chromatography-based liquid chromatography-mass spectrometry[4].
In vitro, C24:1 Ceramide (1μM) treatment of ependymal cells for 48h induced cell ciliary elongation[5].
References:
[1] Manni M M, Sot J, Arretxe E, et al. The fatty acids of sphingomyelins and ceramides in mammalian tissues and cultured cells: Biophysical and physiological implications[J]. Chemistry and physics of lipids, 2018, 217: 29-34.
[2] Birbes H, El Bawab S, Obeid L M, et al. Mitochondria and ceramide: intertwined roles in regulation of apoptosis[J]. Advances in enzyme regulation, 2002, 42: 113-129.
[3] Nagata M, Izumi Y, Ishikawa E, et al. Intracellular metabolite β-glucosylceramide is an endogenous Mincle ligand possessing immunostimulatory activity[J]. Proceedings of the National Academy of Sciences, 2017, 114(16): E3285-E3294.
[4] Von Gerichten J, Schlosser K, Lamprecht D, et al. Diastereomer-specific quantification of bioactive hexosylceramides from bacteria and mammals [S][J]. Journal of lipid research, 2017, 58(6): 1247-1258.
[5] Kong J N, Hardin K, Dinkins M, et al. Regulation of Chlamydomonas flagella and ependymal cell motile cilia by ceramide-mediated translocation of GSK3[J]. Molecular biology of the cell, 2015, 26(24): 4451-4465.
| Cell experiment [1]: | |
Cell lines | Ependymal cells |
Preparation Method | Quantitation of motile cilium length in ependymal cells treated for 48h with ceramide biosynthesis inhibitors (5μM myriocin, 10μM FB1) with or without exogenous C24:1 ceramide (1μM). |
Reaction Conditions | 1μM; 48h |
Applications | Addition of C24:1 ceramide to the culture medium rescued cilia in cells treated with FB1 and induced elongation of cilia in cells not treated with ceramide biosynthesis inhibitors. |
References: | |
| Cas No. | 54164-50-0 | SDF | |
| المرادفات | Ceramide (d18:1/24:1(15Z)); Cer(d18:1/24:1(15Z)); Nervonic Ceramide; N-Nervonoyl-D-erythro-Sphingosine | ||
| Chemical Name | N-[(1S,2R,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-15Z-tetracosenamide | ||
| Canonical SMILES | OC[C@H](NC(CCCCCCCCCCCCC/C=C\CCCCCCCC)=O)[C@H](O)/C=C/CCCCCCCCCCCCC | ||
| Formula | C42H81NO3 | M.Wt | 648.1 |
| الذوبان | 3mg/ml in ethanol; <20ug/ml in DMSO; >5.5mg/ml in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.543 mL | 7.7149 mL | 15.4297 mL |
| 5 mM | 0.3086 mL | 1.543 mL | 3.0859 mL |
| 10 mM | 0.1543 mL | 0.7715 mL | 1.543 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)
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