Calpeptin |
| رقم الكتالوجGC10342 |
A calpain inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 117591-20-5
Sample solution is provided at 25 µL, 10mM.
IC50: 5 nM for human calpain 1
Calpeptin is a calpain inhibitor.
As a calcium-dependent intracellular cysteine protease, calpain plays an key role in various cellular processes including cell differentiation, growth and apoptosis. In rheumatoid arthritis patients, over-expression of calpains are observed in arthritic synovial fluid and are thought to play critical roles in various cellular processes.
In vitro: Calpeptin reduced production of TGF-b1, IL-6, angiopoietin-1 and collagen synthesis from lung fibroblasts. Calpeptin also reduced both angiopoietin-1-dependent migration and IL-6-dependent proliferation of the cells, which could be the underlying mechanism of the preventive effect of Calpeptin on pulmonary fibrosis [1].
In vivo: The preventive effect of calpeptin has been examined on bleomycin-induced pulmonary fibrosis in mice. Histological examinations and quantitative measurements of related proteins in bleomycin-treated mouse lung tissues with or without calpeptin were performed. Calpeptin histologically ameliorated bleomycininduced pulmonary fibrosis in mice. Results showed that calpeptin decreased the expression of IL-6, TGF-b1, angiopoietin-1 and collagen type Ia1 mRNA in mouse lung tissues [1].
Clinical trial: N/A
Reference:
[1] Tabata C,Tabata R,Nakano T. The calpain inhibitor calpeptin prevents bleomycin-induced pulmonary fibrosis in mice. Clin Exp Immunol.2010 Dec;162(3):560-7.
| Cas No. | 117591-20-5 | SDF | |
| Chemical Name | benzyl N-[4-methyl-1-oxo-1-(1-oxohexan-2-ylamino)pentan-2-yl]carbamate | ||
| Canonical SMILES | CCCCC(C=O)NC(=O)C(CC(C)C)NC(=O)OCC1=CC=CC=C1 | ||
| Formula | C20H30N2O4 | M.Wt | 362.47 |
| الذوبان | ≥ 87.6 mg/mL in DMSO, ≥ 96.6 mg/mL in EtOH | Storage | Desiccate at 4°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.7588 mL | 13.7942 mL | 27.5885 mL |
| 5 mM | 0.5518 mL | 2.7588 mL | 5.5177 mL |
| 10 mM | 0.2759 mL | 1.3794 mL | 2.7588 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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