Chloramphenicol |
| رقم الكتالوجGC15185 |
Chloramphenicol is a highly lipid-soluble broad-spectrum antibiotic for bacterial infections. Chloramphenicol inhibits protein synthesis by binding to the 50S subunit of the ribosome, thus acting as an antibacterial agent. Chloramphenicol can be used in the treatment of meningitis and cancer research.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 56-75-7
Sample solution is provided at 25 µL, 10mM.
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Chloramphenicol is a highly lipid-soluble broad-spectrum antibiotic for bacterial infections. Chloramphenicol inhibits protein synthesis by binding to the 50S subunit of the ribosome, thus acting as an antibacterial agent. Chloramphenicol can be used in the treatment of meningitis and cancer research[1-3].
Chloramphenicol inhibits the oxygen destabilizing transcription factor hypoxia-inducible factor 1α (HIF-1α) in hypoxic A549 and H1299 cells, and also inhibits mRNA levels of vascular endothelial growth factor and glucose transporter protein 1[1]. Chloramphenicol induces the expression of matrix metalloproteinase (MMP)-13, which leads to increased cancer cell invasion.Chloramphenicol activates c-Jun N-terminal kinase (JNK) and PI3K/Akt signaling, leading to the phosphorylation of c-Jun protein, and the activated c-Jun protein activates binding to the MMP-13 promoter and upregulate MMP-1 levels[2].
In MPTP-induced Parkinson's (PD) mouse models, Chloramphenicol (50 mg/kg) attenuates toxin-induced dopaminergic neuronal loss by blocking the target site of paraquat (PQ) action[3]. Chloramphenicol can inhibit the increase of body weight and liver weight induced by piperidol oxide (4-OH-tempo) and allopurinol (AP) in mice, and at the same time, chloramphenicol can induce a significant increase in lipid peroxidation during the formation of liver giant mitochondria[4].
References:
[1] Hsu HL, Liao PL, Cheng YW, Huang SH, Wu CH, Li CH, Kang JJ. Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells. Int J Mol Sci. 2019 Jan 3;20(1):157.
[2] Li CH, Cheng YW, Liao PL, Yang YT, Kang JJ. Chloramphenicol causes mitochondrial stress, decreases ATP biosynthesis, induces matrix metalloproteinase-13 expression, and solid-tumor cell invasion. Toxicol Sci. 2010 Jul;116(1):140-50.
[3] Han J, Kim S J, Ryu M J, et al. Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin‐Induced Parkinson’s Disease Model[J]. Oxidative Medicine and Cellular Longevity, 2019, 2019(1): 4174803.
[4] Matsuhashi T, Liu X R, Nishizawa Y, et al. Mechanism of the formation of megamitochondria in the mouse liver induced by chloramphenicol[J]. Toxicology letters, 1996, 86(1): 47-54.
| Cell experiment [1]: | |
Cell lines | A549 and H1299 cells |
Preparation Method | After hypoxic A549 and H1299 cells were incubated with chloramphenicol for 3 h, the expression level of HIF-1α protein was detected by western blot. |
Reaction Conditions | 1-100μg/mL, 3 h |
Applications | Chloramphenicol inhibited the accumulation of HIF-1α protein in A549 and H1299 cells in a concentration-dependent manner. |
| Animal experiment [2]: | |
Animal models | Parkinson's mouse model |
Preparation Method | Mice were divided into three groups (n = 5): group 1, vehicle control; group 2, vehicle and (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) MPTP 20 mg/kg i.p.; group 3 was treated MPTP 20 mg/kg i.p. with Chloramphenicol at a dose of 50 mg/kg at 3 times by oral gavage (-1, 0, and 1 day based on MPTP injection). |
Dosage form | 50mg/kg, 3 days, i.g. |
Applications | Chloramphenicol prevents dopaminergic neuronal loss in the nigral pathway in the MPTP-induced PD mouse model. |
References: | |
| Cas No. | 56-75-7 | SDF | |
| Chemical Name | 2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide | ||
| Canonical SMILES | C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-] | ||
| Formula | C11H12Cl2N2O5 | M.Wt | 323.13 |
| الذوبان | ≥ 16.16 mg/mL in DMSO, ≥ 16.25 mg/mL in Water with ultrasonic and warming, ≥ 33 mg/mL in EtOH | Storage | Store at 2-8°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0947 mL | 15.4736 mL | 30.9473 mL |
| 5 mM | 0.6189 mL | 3.0947 mL | 6.1895 mL |
| 10 mM | 0.3095 mL | 1.5474 mL | 3.0947 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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