Cy3-dUTP (Synonyms: Aminoallyl-dUTP - Cy3; 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy3, Triethylammonium salt; Cy3 dUTP) |
| رقم الكتالوجGC16524 |
Direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Cy3-dUTP can be used for direct enzymatic labeling of DNA/cDNA by PCR with Taq polymerase, Nick Translation with DNAse I/ DNA Polymerase I, cDNA labeling and 3’-end labeling. Cy3-dUTP can replace TTP in reactions in which it serves as a substrate for T4 and Taq DNA polymerases, E. coli DNA polymerase (holoenzyme and Klenow fragment), reverse transcriptase (from AMV and M-MuLV) and terminal transferase. The synthesized dye-labeled DNA/cDNA probes can be used for the identification of specific sequences by in-situ hybridization, microarray or blotting techniques. Cy3-dUTP can also be used for multicolor fluorescence labeling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Cy3-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Cy3-dUTP/50% dTTP.
Average Rating: 5 (Based on Reviews and 17 reference(s) in Google Scholar.)
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