الصفحة الرئيسية>>Labeling & Detection>> Cyanine Dyes>>Cy5 Boc-hydrazide (non-sulfonated)

Cy5 Boc-hydrazide (non-sulfonated)

رقم الكتالوجGC10748

carbonyl-reactive dye

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Cy5 Boc-hydrazide (non-sulfonated) التركيب الكيميائي

الحجم السعر المخزون الكميّة
5mg
157٫00
متوفر
25mg
320٫00
متوفر

Tel:(909) 407-4943 Email: sales@glpbio.com


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Sample solution is provided at 25 µL, 10mM.

Description of Cy5 Boc-hydrazide (non-sulfonated)

Boc-protected Cy5 hydrazide is a reactive dye for the labeling of aldehydes and ketones with high aqueous solubility. Trifluoroacetic acid or other strong acid can remove the Boc-(tert-butoxycarbonyl) protective group. Reactive hydrazide can couple with various carbonyl groups yielding Cy5-labeled molecules. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

Chemical Properties of Cy5 Boc-hydrazide (non-sulfonated)

Cas No. SDF
Chemical Name 1-(6-(2-(tert-butoxycarbonyl)hydrazinyl)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium
Canonical SMILES CC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(NNC(OC(C)(C)C)=O)=O)C4=CC=CC=C4C3(C)C
Formula C37H49ClN4O3 M.Wt 633.26
الذوبان good in dichloromethane, polar organic solvents (DMF, DMSO), alcohols, low in water Storage 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Cy5 Boc-hydrazide (non-sulfonated)

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.5791 mL 7.8957 mL 15.7913 mL
5 mM 0.3158 mL 1.5791 mL 3.1583 mL
10 mM 0.1579 mL 0.7896 mL 1.5791 mL
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In vivo Formulation Calculator (Clear solution) of Cy5 Boc-hydrazide (non-sulfonated)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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