Cy7 NHS ester |
| رقم الكتالوجGC16967 |
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Sulfo-Cy7 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. This reagent allows prepare Cy7-labeled biomolecules, especially useful for the labeling of delicate proteins, and proteins prone to denaturation. Dye labeled molecules can be used for kinds of researches related experiments. Near infrared fluorescent imaging takes advantage of transparency of biological tissues at particular range of wavelengths. In live organisms, the method is non-destructive, and allows monitor distribution of various labeled molecules. Non-sulfonated Cy7 NHS ester soluble in organic phase is also available. For labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In mice, Cy7-labeled RIG precursor solution and Cy7-labeled PIC micelles solution, was analyzed with an IVIS spectrum system. Administration of the Cy7-labeled RIG resulted in strong fluorescence signal in the mouth as compared with the orally administered Cy7-labeled PIC micelles solution [1].
Reference:
[1] Saita, M. ; Kaneko, J.; Sato, T.; Takahashi, S.; Takahashi, S.W.; Kawamata, R.; Sakurai, T.; Lee, M.-C.; Hamada, N.; Kimoto, K.; Nagasaki, Y. Novel antioxidative nanotherapeutics in a rat periodontitis model: Reactive oxygen species scavenging by redox injectable gel suppresses alveolar bone resorption. Biomaterials, 2016, 76, 292–301.
| Cas No. | SDF | ||
| Chemical Name | (2E)-1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]indole-5-sulfonate | ||
| Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC3=CC(=CC=C4C(C5=C(N4CCCCCC(=O)ON6C(=O)CCC6=O)C=CC(=C5)S(=O)(=O)[O-])(C)C)CCC3)C)C | ||
| Formula | C41H46N3NaO10S2 | M.Wt | 827.94 |
| الذوبان | good in water, DMF, DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.2078 mL | 6.0391 mL | 12.0782 mL |
| 5 mM | 0.2416 mL | 1.2078 mL | 2.4156 mL |
| 10 mM | 0.1208 mL | 0.6039 mL | 1.2078 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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