Hoechst 33342 analog 2 trihydrochloride |
رقم الكتالوجGC36246 |
Hoechst 33342 analog 2 trihydrochloride هو عبارة عن anglog لـ Hoechst 33342
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Cas No.: 155815-98-8
Sample solution is provided at 25 µL, 10mM.
Hoechst 33342 analog 2 trihydrochloride is an analog of Hoechst 33342 dye. Hoechst 33342 is a nuclear dye that binds to the grooves in the DNA double strands. Hoechst 33342 dye is also used to differentiate between normal cells, apoptotic cells, and necrotic cells. The nuclei of normal cells are round and uniformly stained, but during mitosis, the nuclei are also condensed, and DNA forms two parallel lines when chromosomes are separated; in apoptotic cells, due to the condensation of DNA, the nuclei are usually fragmented and stained more Strong; the DNA in necrotic cells is not concentrated, and the edges of the nucleus are unclear[1].
Hoechst dyes can also be used to monitor cell viability by tracking changes in their emission spectra. As slightly groove-binding DNA stains with AT selectivity, Hoechst dyes are able to bind to all nucleic acids, but they show greater fluorescence enhancement for AT-rich double-stranded DNA strands compared to GC-rich strands[2]. This property has been used to identify Q bands in chromosomes, which are AT base pair-rich regions that fluoresce brightly when stained with quinacrine dye[3].
References:
[1]. Lisa C Crowley, Brooke J Marfell , Nigel J Waterhouse. Analyzing Cell Death by Nuclear Staining with Hoechst 33342. 2016 Sep 1;2016(9). doi: 10.1101/pdb.prot087205.
[2]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4′, 6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression. 1988 Feb 28;949(2):158-68.
[3]. Weisblum B, Haenssler E. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA. Chromosoma. 1974 Sep;46(3):255-60.
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