Katacalcin (PDN 21) |
| رقم الكتالوجGC33793 |
كاتاكالسين (PDN 21) (PDN 21) هو ببتيد فعال في خفض الكالسيوم في البلازما.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 85916-47-8
Sample solution is provided at 25 µL, 10mM.
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Katacalcin is a second potent plasma calcium-lowering peptide.
Katacalcin is a potent plasma calcium lowering peptide. Katacalcin belongs to the calcitonin family, that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of these ions in the bones[1]. Katacalcin (KC) belongs to a small family of polypeptides that are encoded by the calc-1 gene and also include calcitonin (CT) and procalcitonin NH2-terminal cleavage peptide (N-ProCT). Katacalcin pretreatment leads to a concentration-dependent decrease at concentrations between 1 amol/liter and 10 fmol/liter and is a more potent inhibitor of fMLP-induced chemotaxis than CT or procalcitonin (PCT). Katacalcin deactivates CD14+ peripheral blood mononuclear cell (PBMC) chemotaxis not only toward N-formyl-Met-Leu-Phe (fMLP) but also toward other attractants of the chemokine family (heterologous deactivation) as well as toward PCT and CT. Pretreatment of CD14+ PBMCs with Katacalcin also deactivates subsequent chemotaxis toward Katacalcin itself. Katacalcin elicites concentration-dependent migration of CD14+ PBMC at concentrations from the atomolar to the micromolar range and deactivates attractant-induced chemotaxis. Katacalcin regulates human CD14+ PBMC migration via signaling events involving protein kinase A-dependent cAMP pathways[2].
[1]. Hillyard CJ, et al. Katacalcin: a new plasma calcium-lowering hormone. Lancet. 1983 Apr 16;1(8329):846-8. [2]. Kaneider NC, et al. Involvement of cyclic adenosine monophosphate-dependent protein kinase A and pertussis toxin-sensitive G proteins in the migratory response of human CD14+ mononuclear cells tokatacalcin. J Bone Miner Res. 2002 Oct;17(10):1872-82.
Kinase experiment: | Leukocyte migration is measured using a modified 48-blind well microchemotaxis chamber equipped with 5 μm pore-sized nitrocellulose filters for CD14+ PBMC chemotaxis. In some experiments cells are incubated for 20 minutes with GFX (500 nM), Staurosporine (10 ng/mL), Tyrphostin-23 (10 ng/mL), Wortmannin (WTN) (10 nmol/liter), Protein kinase A inhibitor (PKI) (from 0.1 to 100 ng/mL) or Rp-cAMPS (from 100 pM to 100 μM), or CTX (1 nM) or pertussis toxin (PTX) (1 nM). For determination of Katacalcin 's potency to deactivate CD14+ PBMC chemotaxis toward fMLP, cells are incubated with Katacalcin (from 1 amol/liter to 1 μmol/liter) for 20 minutes. For control, cAMP-independent migration of CD14+ PBMC toward bombesin is tested in some of the experiments. After washing twice, 50 μL of a cell suspension (1×106 cells/mL) is put into the upper compartment of the chemotaxis chamber and cells are allowed to migrate for 90 minutes toward peptides derived from the calc-1 gene in the lower wells. After these migration periods, the filters are dehydrated, fixed, and stained with hematoxylin and eosin. Migration depth is quantified by microscopy, measuring the distance from the surface of the filter to the leading front of three cells migration[2]. |
References: [1]. Hillyard CJ, et al. Katacalcin: a new plasma calcium-lowering hormone. Lancet. 1983 Apr 16;1(8329):846-8. | |
| Cas No. | 85916-47-8 | SDF | |
| Canonical SMILES | Asp-Met-Ser-Ser-Asp-Leu-Glu-Arg-Asp-His-Arg-Pro-His-Val-Ser-Met-Pro-Gln-Asn-Ala-Asn | ||
| Formula | C97H154N34O36S2 | M.Wt | 2436.6 |
| الذوبان | Soluble in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.4104 mL | 2.052 mL | 4.1041 mL |
| 5 mM | 0.0821 mL | 0.4104 mL | 0.8208 mL |
| 10 mM | 0.041 mL | 0.2052 mL | 0.4104 mL |
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- Purity: >98.00%
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