Leishman's stain |
| رقم الكتالوجGC66482 |
بقعة Leishman'؛ s هي أداة تلطيخ أساسية لتلطيخ الدم المحيطي ومسحات نخاع العظام (تظهر باللون الرمادي الباهت المزرق إلى الأزرق الغامق تحت عدسة الغمر بالزيت).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 12627-53-1
Sample solution is provided at 25 µL, 10mM.
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Leishman's stain is an essential staining tool for for staining of the peripheral blood and bone marrow smears (displayed pale bluish-grey to deep blue under oil-immersion lens)[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Prepare stock solution of Sorensen's buffer: Solution A. Na2HPO4•2H2O (9.5 g/L); Solution B. KH2PO4 (9.1 g/L).
2. Cover the smear with Leishman stain for 2 min.
3. Add double volume of Sorensen buffer (6.8 pH) to the slide and mix gently for 15 min.
4. Wash off with running water and dry the smear (5 min).
[1]. S Gajendra, et al. Leishman and Giemsa stain: a new reliable staining technique for blood/bone marrow smears. Int J Lab Hematol. 2015 Dec;37(6):774-82.
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