Monochlorobimane (Chlorobimane) (Synonyms: Chlorobimane) |
رقم الكتالوجGC30550 |
Monochlorobimane (Chlorobimane) (Chlorobimane) عبارة عن صبغة فلورية (&Lambda ؛ مثال = 380 نانومتر ، & لامدا ؛ em = 470 نانومتر) لقياس الجلوتاثيون (GSH) في المقايسات الخلوية.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 76421-73-3
Sample solution is provided at 25 µL, 10mM.
Monochlorobimane is a fluorescent dye (λex=380 nm, λem=470 nm).
A common technique to measure Glutathione (GSH) in cultured cells is to add Monochlorobimane to the culture medium where it readily enters cells to form a fluorescent GSH-monochlorobimane adduct that can be measured fluorometrically. It is clear that the Monochlorobimane approach gives comparable results to the HPLC technique. Although most laboratories use 100 mM Monochlorobimane in their assay our results show that the Monochlorobimane concentration can be lowered to 25 mM and still accurately measure homogenate GSH content[1].
[1]. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7.
Average Rating: 5
(Based on Reviews and 21 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *