NSC 319726 (Synonyms: ZMC1) |
| رقم الكتالوجGC15404 |
A p53 reactivator
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 71555-25-4
Sample solution is provided at 25 µL, 10mM.
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NSC 319726 is a reactivator of mutant p53 with IC50 value of 8nM [1].
NSC 319726 belongs to the thiosemicarbazone family. In a mouse fibroblast cell line Balb/c 3T3, NSC 319726 shows greater inhibition of cell growth in cells expressing mutant p53 than in WT p53 control. NSC 319726 is also nontoxic to WI38 human fibroblasts (p53 WT). In three different ovarian carcinoma cell lines (TOV112D (p53R175H), OVCAR3 (p53R248W) and SKOV3 (p53-/-)), treatment of NSC 319726 results in an induction of apoptosis in the p53R175 mutant. NSC 319726 restores p53 function through inducing a conformation change of mutant p53 to a “WT-like” structure which can be recognized by the WT specific antibody. The inhibition of cell growth caused by NSC 319726 is also found in the TOV112D (p53R175H) xenografts when the dose of NSC 319726 is 1mg/kg. Furthermore, it is shown that zinc ion chelation and ROS changes are important for the apoptosis induced by NSC319726 [1].
References:
[1] Yu X, Vazquez A, Levine AJ, Carpizo DR. Allele-specific p53 mutant reactivation. Cancer Cell. 2012 May 15;21(5):614-25.
| Cas No. | 71555-25-4 | SDF | |
| المرادفات | ZMC1 | ||
| Chemical Name | N-[(Z)-1-pyridin-2-ylethylideneamino]azetidine-1-carbothioamide | ||
| Canonical SMILES | CC(=NNC(=S)N1CCC1)C2=CC=CC=N2 | ||
| Formula | C11H14N4S | M.Wt | 234.32 |
| الذوبان | ≥ 8.1 mg/mL in DMSO, ≥ 3.43 mg/mL in EtOH with ultrasonic and warming | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.2677 mL | 21.3383 mL | 42.6767 mL |
| 5 mM | 0.8535 mL | 4.2677 mL | 8.5353 mL |
| 10 mM | 0.4268 mL | 2.1338 mL | 4.2677 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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