Protease and Phosphatase Inhibitor Cocktail (EDTA Free, 100X in ddH2O)

This product is a ready-to-use mixture of protease and phosphatase inhibitors (EDTA-free, 100X in ddH2O), which can effectively inhibit the activity of proteases and phosphatases in conventional cell lysis and protein extraction. This product is suitable for experiments such as protein extraction from mammalian cells, primary cells, yeast cells, bacteria, animal tissues and plant tissues.
The main ingredients of this product include: serine|cysteine protease inhibitor Leupeptin, bovine trypsin inhibitor Aprotinin, aminopeptidase inhibitor Bestatin, cysteine protease inhibitor E-64, serine|threonine phosphatase reversible inhibitor β-glycerophosphate, serine|threonine phosphatase irreversible inhibitor Sodium Pyrophosphate, acid phosphatase inhibitor Sodium Fluoride, alkaline phosphatase|tyrosine phosphatase inhibitor Sodium Orthovanadate. In addition, this product does not contain EDTA, and metalloproteinases and aspartic acid proteases are not inhibited.
This product can effectively inhibit the functions of proteases such as serine proteases, leucine aminopeptidase, cysteine protease and aminopeptidase B, as well as phosphatases such as serine|threonine phosphatases, protein tyrosine phosphatases, acid phosphatases and alkaline phosphatases.
Recommended usage: When lysing cells or extracting proteins, mix this product with lysis buffer at a volume ratio of 1:100.