PtdIns-(3,4,5)-P3 (1,2-dipalmitoyl)-d62 (sodium salt) (Synonyms: DPPI3,4,5P3d62, Phosphatidylinositol3,4,5trisphosphate C16d62)

PtdIns-(3,4,5)-P₃ (1,2-dipalmitoyl)-d₆₂ (sodium salt) contains 62 deuterium atoms at the 2, 2', 3, 3', 4, 4', 5, 5', 6, 6', 7, 7', 8, 8', 9, 9', 10, 10', 11, 11', 12, 12', 13, 13', 14, 14', 15, 15', 16, 16, and 16 positions of each fatty acyl chain. It is intended for use as an internal standard for the quantification of PtdIns-(3,4,5)-P₃ (1,2-dipalmitoyl) (sodium salt) by GC- or LC-mass spectrometry (MS). The phosphatidylinositol phosphates represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.^1,2 PtdIns-(3,4,5)-P₃ can serve as an anchor for the binding of signal transduction proteins bearing pleckstrin homology (PH) domains. Centuarin α and the Akt-family of GTPase activating proteins are examples of PtdIns-(3,4,5)-P₃-binding proteins.^3,4 Protein-binding to PtdIns-(3,4,5)-P₃ is important for cytoskeletal rearrangements and membrane trafficking. PtdIns-(3,4,5)-P₃ is resistant to cleavage by PI-specific phospholipase C (PLC). Thus, it is likely to function in signal transduction as a modulator in its own right, rather than as a source of inositol tetraphosphates. For further reading on inositol phospholipids, see references ⁵ and ⁶.

1.Lapetina, E.G., Billah, M.M., and Cuatrecasas, P.The phosphatidylinositol cycle and the regulation of arachidonic acid productionNature292367-369(1981) 2.Majerus, P.W.Inositol phosphate biochemistryAnnu. Rev. Biochem.61225-250(1992) 3.Tanaka, K., Imajoh-Ohmi, S., Sawada, T., et al.A target of phosphatidylinositol 3,4,5-trisphosphate with a zinc finger motif similar to that of the ADP-ribosylation-factor GTPase-activating protein and two pleckstrin homology domainsEuropean Journal of Biochemistry245512-519(1997) 4.Yang, X., Rudolf, M., Carew, M.A., et al.Inositol 1,3,4-trisphosphate acts in vivo as a specific regulator of cellular signaling by inositol 3,4,5,6-tetrakisphosphateThe Journal of Biological Chemisty27418973-18980(1999) 5.Pike, L.J., and Casey, L.Localization and turnover of phosphatidylinositol 4,5-bisphosphate in caveolin-enriched membrane domainsThe Journal of Biological Chemisty27126453-26456(1996) 6.Berridge, M.J.Inositol trisphosphate and calcium signallingNature361(6410)315-325(1993)