WSP-1 (Synonyms: Washington State Probe1) |
رقم الكتالوجGC45161 |
WSP-1 هو مؤشر فلوري انتقائي وسريع الاستجابة للكبريتيدات النشطة، صمم خصيصًا للكشف عن كبريتيد الهيدروجين (H2S) داخل العينات الحية والبيئات داخل الخلايا.
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Cas No.: 1352750-34-5
Sample solution is provided at 25 µL, 10mM.
WSP-1 is a selective and rapid responsive fluorescent probe for active sulfides, specifically designed for the detection of hydrogen sulfide (H2S) within biological samples and intracellular environments. WSP-1 selectively and rapidly reacts with H2S, generating benzodithiolone and a fluorescent compound (Ex=465nm, Em=515nm) [1].
WSP-1 (10 µM) can detect sulfide levels in RFL-6 cells where the sulfide donor GYY4137 (10-100 µM) is present[2]. The use of the WSP-1 fluorescent dye enables the detection of hydrogen sulfide (H2S) generation in human vascular smooth muscle cells following H2S donor administration[3]. WSP-1 can applied to track endogenous H2S in tomato (Solanum lycopersicum) roots in site[4].
References:
[1]. Liu C, Pan J, et,al. Capture and visualization of hydrogen sulfide by a fluorescent probe. Angew Chem Int Ed Engl. 2011 Oct 24;50(44):10327-9. doi: 10.1002/anie.201104305. Epub 2011 Sep 6. PMID: 21898737; PMCID: PMC3417056.
[2]. Cortese-Krott MM, Fernandez BO, et,al. Nitrosopersulfide (SSNO(-)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide. Redox Biol. 2014 Jan 11;2:234-44. doi: 10.1016/j.redox.2013.12.031. PMID: 24494198; PMCID: PMC3909780.
[3]. Martelli A, Citi V,et,al. Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells. Methods Mol Biol. 2019;2007:79-87. doi: 10.1007/978-1-4939-9528-8_6. PMID: 31148107.
[4]. Li YJ, Chen J, et,al. In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation. PLoS One. 2014 Feb 27;9(2):e90340. doi: 10.1371/journal.pone.0090340. PMID: 24587333; PMCID: PMC3937356.
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