Baicalin (Synonyms: Baicalein 7-glucuronide) |
| Catalog No.GN10018 |
Baicalin is a flavonoid glycoside and an allosteric carnitine palmitoyltransferase 1 (CPT1) activator.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 21967-41-9
Sample solution is provided at 25 µL, 10mM.
Baicalin is a flavonoid glycoside and an allosteric carnitine palmitoyltransferase 1 (CPT1) activator[1]. Baicalin can inhibit the replication of human immunodeficiency virus type 1 (HIV-1)[2]. Baicalin can reduce NF-κB expression and mediate the regulation of key cancer signaling pathways[3].
In vitro, Baicalin (0.005-0.5nM) treatment of RAW264.7 cells inhibited LPS-induced cell proliferation and reduced the expression of intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1), and cyclooxygenase-2 (Cox-2) proteins[4]. Baicalin (100μM) pretreatment of HK-2 cells for 1h can improve cell viability after H2O2 stimulation, reduce oxidative stress, and inhibit caspase-3 activation and cell apoptosis[5].
In vivo, Baicalin (10, 100 mg/kg) was intraperitoneally injected into rats with renal ischemia-reperfusion injury (IRI), which reduced oxidative stress and histological damage, improved renal function, inhibited proinflammatory response and tubular apoptosis, and reduced the expression of TLR2, TLR4, MyD88, p-NF-κB and p-IκB proteins and caspase-3 activity, and increased the Bcl-2/Bax ratio[6]. Baicalin (400 mg/kg) was intraperitoneally injected into diabetic nephropathy (DN) mice, which effectively improved diabetic conditions, proteinuria, renal histopathological changes and cell apoptosis, and inhibited the activation of the classic proinflammatory signaling pathway MAPK family, such as Erk1/2, JNK and P38MAPK signaling pathways [7].
References:
[1] Dai J, Liang K, Zhao S, et al. Chemoproteomics reveals baicalin activates hepatic CPT1 to ameliorate diet-induced obesity and hepatic steatosis[J]. Proceedings of the National Academy of Sciences, 2018, 115(26): E5896-E5905.
[2] Kitamura K, Honda M, Yoshizaki H, et al. Baicalin, an inhibitor of HIV-1 production in vitro[J]. Antiviral research, 1998, 37(2): 131-140.
[3] Yu X, Liu Y, Wang Y, et al. Baicalein induces cervical cancer apoptosis through the NF-κB signaling pathway[J]. Molecular Medicine Reports, 2018, 17(4): 5088-5094.
[4] Cui L, Feng L, Zhang Z H, et al. The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation[J]. International Immunopharmacology, 2014, 23(1): 294-303.
[5] Lin M, Li L, Zhang Y, et al. Baicalin ameliorates H2O2 induced cytotoxicity in HK-2 cells through the inhibition of ER stress and the activation of Nrf2 signaling[J]. International journal of molecular sciences, 2014, 15(7): 12507-12522.
[6] Lin M, Li L, Li L, et al. The protective effect of baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis[J]. BMC complementary and alternative medicine, 2014, 14: 1-9.
[7] Ma L, Wu F, Shao Q, et al. Baicalin alleviates oxidative stress and inflammation in diabetic nephropathy via Nrf2 and MAPK signaling pathway[J]. Drug design, development and therapy, 2021: 3207-3221.
| Cell experiment [1]: | |
Cell lines | RAW264.7 cells |
Preparation Method | Cells were treated with 0.005-0.5nM of Baicalin and LPS (1 μg/ml). MTT solution of 10μl(5mg/ml) was added to each well for 4 h. At the end of incubation, DMSO of 100μl was added for 10 min after the removal of medium. |
Reaction Conditions | 0.005-0.5nM; 4h |
Applications | Exposure to 1μg/ml LPS significantly increased the cell proliferation, Baicalin had an inhibition on LPS-induced RAW264.7 cell proliferation. |
| Animal experiment [2]: | |
Animal models | Male Wistar rats |
Preparation Method | Rats were randomly divided into five groups of six rats each, renal IRI was induced by clamping the left renal artery for 45 min plus a right nephrectomy. Saline-treated animals received intraperitoneal injections of 1mL 0.9% sterile NaCl 30 min before renal clamping. Baicalin-treated rats received intraperitoneal injections of Baicalin, diluted in sterile saline to 1, 10, or 100 mg/kg body weight 30 min before renal clamping. |
Dosage form | 1, 10, or 100 mg/kg; i.p. |
Applications | Baicalin treatment decreased oxidative stress and histological injury, and improved kidney function, as well as inhibiting proinflammatory responses and tubular apoptosis. |
References: [1] Cui L, Feng L, Zhang Z H, et al. The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation[J]. International Immunopharmacology, 2014, 23(1): 294-303. [2] Lin M, Li L, Li L, et al. The protective effect of baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis[J]. BMC complementary and alternative medicine, 2014, 14: 1-9. | |
| Cas No. | 21967-41-9 | SDF | |
| Synonyms | Baicalein 7-glucuronide | ||
| Chemical Name | (2S,3S,4S,5R,6S)-6-(5,6-dihydroxy-4-oxo-2-phenylchromen-7-yl)oxy-3,4,5-trihydroxyoxane-2-carboxylic acid | ||
| Canonical SMILES | C1=CC=C(C=C1)C2=CC(=O)C3=C(C(=C(C=C3O2)OC4C(C(C(C(O4)C(=O)O)O)O)O)O)O | ||
| Formula | C21H18O11 | M.Wt | 446.37 |
| Solubility | DMSO: ≥ 100 mg/mL (224.03 mM） | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2403 mL | 11.2015 mL | 22.4029 mL |
| 5 mM | 0.4481 mL | 2.2403 mL | 4.4806 mL |
| 10 mM | 0.224 mL | 1.1201 mL | 2.2403 mL |
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Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
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Related Biological Data
Baicalin prevented ΔΨm loss and mitochondrial ROS generation that BLM-induced in MLE-12, 5-HD blocked the protective effect. (A) MitoTracker (red) probe indicated the change of mitochondrial membrane potential changes in MLE-12 cells.
For experiments, cells were seeded in plates and grown to 60%− 70% confluency before they were treated with 5-Hydroxydecanoate sodium was (100μM) for 30min, diazoxide (100μM) or baicalin (90μM，GLPBIO, USA) for 30min.
Toxicology (2023): 153638. PMID: 37783230 IF: 4.5003 -
Related Biological Data
Baicalin inhibited expression of FTH1 in OSCC cells. (I, J) Western blot analysis of FTH1 expression changes after overexpression and baicalin treatment.
The CCK8 assay was used to detect the cell viability of Cal27 and SCC25 cells treated with baicalin (GLPBIO, USA) at 0, 5, 10, 20, 4, and 60μM for 24h.
The Journal of Gene Medicine 26.2 (2024): e3669. PMID: 38380717 IF: 3.5
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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