Brassinazole |
| Catalog No.GC19544 |
Brassinazole (Brz) is a specific inhibitor of C-22 hydroxylase (CYP90B1) in brassinosteroid (BR) biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs.
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Cas No.: 224047-41-0
Sample solution is provided at 25 µL, 10mM.
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Brassinazole (Brz) is a specific inhibitor of C-22 hydroxylase (CYP90B1) in brassinosteroid (BR) biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs[1]. Brassinazole has regulatory roles in G-fiber cell wall development and secondary xylem cell wall carbohydrate biosynthesis during tension wood formation in hybrid poplar[2]. In addition, brassinazole treatment (5µM) can delay persimmon fruit ripening and suppress respiration rate[3].
Brassinazole treatment (5, 1, and 0.5μM) markedly caused dwarfism of Arabidopsis seedlings in a concentration-dependent manner. Moreover, brassinazole treatment (1μM) showed a BR-deficient mutant-like phenotype, which is rescued by the application of brassinolide (BL) (10nM). Dark-grown 10-d-old Arabidopsis thaliana seedlings were treated with different concentrations of brassinozoles from 0 to 10μM . As the concentration of brassinozole increased, hypocotyl length decreased. The change in length occurred between 0.1 and 0.5μM[4].
[1]. Oh K, Matsumoto T, Yamagami A, Ogawa A, Yamada K, Suzuki R, et al. YCZ-18 is a new brassinosteroid biosynthesis inhibitor. PLoS One. 2015;10(3):e0120812. Epub 20150320. doi: 10.1371/journal.pone.0120812. PubMed PMID: 25793645.
[2]. Gao J, Yu M, Zhu S, Zhou L, Liu S. Effects of exogenous 24-epibrassinolide and brassinazole on negative gravitropism and tension wood formation in hybrid poplar (Populus deltoids X Populus nigra). Planta. 2019;249(5):1449-63. Epub 20190128. doi: 10.1007/s00425-018-03074-2. PubMed PMID: 30689054.
[3]. He Y, Li J, Ban Q, Han S, Rao J. Role of Brassinosteroids in Persimmon ( Diospyros kaki L.) Fruit Ripening. J Agric Food Chem. 2018;66(11):2637-44. Epub 20180312. doi: 10.1021/acs.jafc.7b06117. PubMed PMID: 29509414.
[4]. Asami T, Min YK, Nagata N, Yamagishi K, Takatsuto S, Fujioka S, et al. Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor. Plant Physiol. 2000;123(1):93-100. doi: 10.1104/pp.123.1.93. PubMed PMID.
| Cas No. | 224047-41-0 | SDF | |
| Chemical Name | β-[(4-chlorophenyl)methyl]-α-methyl-α-phenyl-1H-1,2,4-triazole-1-ethanol | ||
| Canonical SMILES | ClC(C=C1)=CC=C1CC(C(C)(O)C2=CC=CC=C2)N3N=CN=C3 | ||
| Formula | C18H18ClN3O | M.Wt | 327.8 |
| Solubility | DMF: 100 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 50 mg/ml,Ethanol: 25 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0506 mL | 15.2532 mL | 30.5064 mL |
| 5 mM | 0.6101 mL | 3.0506 mL | 6.1013 mL |
| 10 mM | 0.3051 mL | 1.5253 mL | 3.0506 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)
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