1-Palmitoyl-d9-2-Palmitoyl-sn-glycero-3-PE MaxSpec® Standard (Synonyms: 16:0-d9/16:0-PE, 1-Hexadecanoyl-d9-2-Hexadecanoyl-sn-glycero-3-Phosphatidylethanolamine, 1-Hexadecanoyl-d9-2-Hexadecanoyl-sn-glycero-3-Phosphoethanolamine, PE(16:0-d9/16:0))

1-Palmitoyl-d₉-2-palmitoyl-sn-glycero-3-PE is intended for use as an internal standard for the quantification of 1,2-dipalmitoyl-sn-glycero-3-PE by GC- or LC-MS. 1,2-Dipalmitoyl-sn-glycero-3-PE (1,2-DPPE) is a naturally occurring PE containing C16:0 fatty acids at the sn-1 and sn-2 positions. It belongs to a class of phospholipids that are the most abundant lipids in the inner leaflet of the plasma membrane.¹ 1,2-DPPE interacts with cholesterol to form a condensed lipid monolayer with tight hydrogen bonding of the 1,2-DPPE interheadgroups, resulting in a more fluid membrane that may aid in transport and signaling across the bilayer.^2,3

1-Palmitoyl-d₉-2-palmitoyl-sn-glycero-3-PE MaxSpec^® standard is a quantitative grade standard of 1-palmitoyl-d₉-2-palmitoyl-sn-glycero-3-PE that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This 1-palmitoyl-d₉-2-palmitoyl-sn-glycero-3-PE MaxSpec^® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

1.Vance, J.E., and Tasseva, G.Formation and function of phosphatidylserine and phosphatidylethanolamine in mammalian cellsBiochim. Biophys. Acta1831(3)543-554(2013) 2.McQuaw, C.M., Sostarecz, A.G., Zheng, L., et al.Lateral heterogeneity of dipalmitoylphosphatidylethanolamine-cholesterol Langmuir-Blodgett films investigated with imaging time-of-flight secondary ion mass spectrometry and atomic force microscopyLangmuir21(3)807-813(2005) 3.Leekumjorn, S., and Sum, A.K.Molecular simulation study of structural and dynamic properties of mixed DPPC/DPPE bilayersBiophys. J.90(11)3951-3965(2006)