2-Aminopurine (hydrochloride) (Synonyms: 2-AP) |
| Katalog-Nr.GC42123 |
2-Aminopurin (Hydrochlorid) ist ein fluoreszierendes Analogon von Guanosin.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1428126-74-2
Sample solution is provided at 25 µL, 10mM.
2-Aminopurine is a fluorescent analog of guanosine and adenosine that is used as a site-specific probe of nucleic acid structure and dynamics. It base pairs with cytosine in a wobble configuration or with thymine in a Watson-Crick geometry. [1] In addition to its usefulness in studies of base stacking interactions, 2-aminopurine has been used as a probe for DNA base flipping by methyl transferases. [2] 2-Aminopurine exhibits an excitation/emission of 320/381 nm, respectively, when adjusted to reduce interference with DNA base absorption as well as tryptophan fluorescence. 2-Aminopurine inhibits the double-stranded RNA-dependent protein kinase, protein kinase R, whose activity mediates antiviral defense and participates in toll-like receptor signaling.[3]
Reference:
[1]. Jean, J.M., and Hall, K.B. 2-Aminopurine fluorescence quenching and lifetimes: Role of base stacking. Proc.Nat.Acad.Sci.USA 98(1), 37-41 (2001).
[2]. Holz, B., Klimasauskas, S., Serva, S., et al. 2-Aminopurine as a fluorescent probe for DNA base flipping by methyltransferases. Nucleic Acids Res. 26(4), 1076-1083 (1998).
[3]. Endoh, Y., Chung, Y.M., Clark, I.A., et al. IL-10-dependent S100A8 gene induction in monocytes/macrophages by double-stranded RNA. J. Immunol. 182(4), 2258-2268 (2009).
| Cas No. | 1428126-74-2 | SDF | |
| Überlieferungen | 2-AP | ||
| Chemical Name | 9H-purin-2-amine, dihydrochloride | ||
| Canonical SMILES | NC1=NC2=NC=NC2=CN1.Cl.Cl | ||
| Formula | C5H5N5•2HCl | M.Wt | 208.1 |
| Löslichkeit | PBS (pH 7.2): 5 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.8054 mL | 24.0269 mL | 48.0538 mL |
| 5 mM | 0.9611 mL | 4.8054 mL | 9.6108 mL |
| 10 mM | 0.4805 mL | 2.4027 mL | 4.8054 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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