3x DYKDDDDK Tag |
Katalog-Nr.GC72182 |
3x DYKDDDDK Tag ist ein nützliches Werkzeug zur Untersuchung der Funktion und Lokalisierung von Proteinen, deren Antikörper (Abs) nicht verfügbar sind.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 921765-76-6
Sample solution is provided at 25 µL, 10mM.
HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].
References:
[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830.
[2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.
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