AAPH |
| Katalog-Nr.GC19496 |
AAPH is a water-soluble azo compound
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2997-92-4
Sample solution is provided at 25 µL, 10mM.
AAPH is a water-soluble azo compound which is used extensively as a free radical generator, often in the study of lipid peroxidation and the characterization of antioxidants.[1],[2],[3],[4] Decomposition of AAPH produces molecular nitrogen and 2 carbon radicals. The carbon radicals may combine to produce stable products or react with molecular oxygen to give peroxyl radicals. The half-life of AAPH is about 175 hours (37°C at neutral pH), making the rate of free radical generation essentially constant during the first several hours in solution.[5] While AAPH may be used effectively for lipid peroxidation in aqueous dispersions of fatty acids, other radical generators may be better suited for peroxidation studies in lipid micelles or membranes.[6],[7]
Reference:[1]. Noguchi, N., Takahashi, M., Tsuchiya, J., et al. Action of 21-aminosteroid U74006F as an antioxidant against lipid peroxidation Biochemical Pharmacology 55, 785-791 (1998).
[2]. Liu, Z.Q., Yu, W., and Liu, Z.L. Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein Chemistry and Physics of Lipids 103, 125-135 (1999).
[3]. Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids Methods in Enzymology 234(24), 279-293 (1994).
[4]. Liégeois, C., Lermusieau, G., and Collin, S. Measuring antioxidant efficiency of wort, malt, and hops against the 2,2'-azobis(2-amidinopropane) dihydrochloride-induced oxidation of an aqueous dispersion of linoleic acid Journal of Agricultural and Food Chemistry 48, 1129-1134 (2000).
[5]. Niki, E. Free radical initiators as source of water- or lipid-soluble peroxyl radicals Methods in Enzymology 186, 100-108 (1990).
[6]. Yamamoto, Y., Haga, S., Niki, E., et al. Oxidation of lipids. V. Oxidation of methyl linoleate in aqueous dispersion Bulletin of the Chemical Society of Japan 57(5), 1260-1264 (1984).
[7]. Culbertson, S.M., and Porter, N.A. Unsymmetrical azo initiators increase efficiency of radical generation in aqueous dispersions, liposomal membranes, and lipoproteins Journal of the American Chemical Society 122, 4032-4038 (2000).
| Cas No. | 2997-92-4 | SDF | |
| Chemical Name | 2,2'-azobis-2-methyl-propanimidamide, dihydrochloride | ||
| Canonical SMILES | N/C(C(C)(C)/N=N/C(C)(C)/C(N)=N\[H])=N/[H].Cl.Cl | ||
| Formula | C8H18N6 • 2HCl | M.Wt | 271.2 |
| Löslichkeit | PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6873 mL | 18.4366 mL | 36.8732 mL |
| 5 mM | 0.7375 mL | 3.6873 mL | 7.3746 mL |
| 10 mM | 0.3687 mL | 1.8437 mL | 3.6873 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
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Then, 5 μL AAPH (GlpBio) (20 mM) was added to the mixture and the reaction continued for 60 min.
Antioxidants 12.3 (2023): 664. PMID: 36978912 IF: 6.9997 -
Related Biological Data
Prevention of KFWD and its fractions on 2,2′ -Azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative DNA damage.
Then, 5 μL AAPH (GlpBio) (50 mM) was added to the mixture and continued the reaction for 60 min.
Toxicon 219 (2022): 106934. PMID: 36195155 IF: 2.8002 -
Related Biological Data
Inhibitory effects of KFEE and its fractions on AAPH-induced oxidative damage of pBR322 DNA. KFEE, the ethanol extract of Kochiae Fructus; ECHF, chloroform fraction; EEAF, ethyl acetate fraction; ENBF, n-butanol fraction; EWF, water fraction.
Then, 4 μL AAPH (GlpBio) was added to the mixture and continued the reaction for 60 min.
Toxicon (2024): 107725. PMID: 38642904 IF: 2.8002
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)
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