Coelenterazine 400a (hydrochloride) |
Katalog-Nr.GC43291 |
Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2320429-05-6
Sample solution is provided at 25 µL, 10mM.
Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).[1],[2] It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h, which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.3 Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.[2],[3] BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.[1],[2],[4]
Reference:
[1]. Bertrand, L., Parent, S., Caron, M., et al. The BRET2/arrestin assay in stable recombinant cells: A platform to screen for compounds that interact with G protein-couple receptors (GPCRS). J. Recept. Signal Transduct. Res. 22(1-4), 533-541 (2002).
[2]. Galés, C., Rebois, R.V., Hogue, M., et al. Real-time monitoring of receptor and G-protein interactions in living cells. Nat. Methods 2(3), 177-184 (2005).
[3]. Pfleger, K.D., and Eidne, K.A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat. Methods 3(3), 165-174 (2006).
[4]. Huang, Q., Acha, V., Yow, R., et al. Bioluminescence measurements in mice using a skin window. J. Biomed. Opt. 12(5):054012, (2007).
Average Rating: 5
(Based on Reviews and 12 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *