Name: Coelenterazine
Brand: GlpBio
SKU: GC17680
Availability: InStock
Rating: 5 (28 reviews)

Product has been cited by 4 publications

Related Biological Data

Superoxide production by HEK293 cells stably expressing NOX5β and COS-7 cells transiently transfected with NOX5β in response to addition of hemin. B) luminescence of coelenterazine
Superoxide levels were also measured using coelenterazine luminescence.Cells were treated as described, but instead of L-012, 50 μM coelenterazine(GlpBio) was added to each well 10 min prior to read.
Redox biology 36 (2020): 101656. PMID: 32738790 IF: 11.793
Related Biological Data

Four crucial factors were optimized for the assay of the LIPS method. S/N was a ratio of the fluorescent value from a positive sample divided by that from a negative control.
The magnetic particles with the complex were suspended in 180 μL hGluc buffer, which consisted of 20 mM Tris-HCl (pH 7.4) and 100 mM NaCl and then the substrate of 5 μL 200 μM coelenterazine(GlpBio) were added to obtain a value of bioluminescence on a microplate reader.
Int J Biol Macromol (2024): 130964. PMID: 38499123 IF: 8.1996
Related Biological Data

Superoxide production by HEK293 cells stably expressing NOX5β and COS-7 cells transiently transfected with NOX5β in response to the NO donor SNAP. B) Superoxide production as measured by luminescence of coelenterazine after 1 hr incubation with DMSO control or 50 μM SNAP.
Radicicol, hemin, NO donor, or Fe(PPIX)NO were added at various timepoints and either 400 μM L-012 or 50 μM coelenterazine(GlpBio) was added 10 min prior to read.
Free Radical Biology and Medicine (2021). PMID: 34139309 IF: 7.373
Related Biological Data

Optimization of the RBD-hGluc LIPS assay conditions. Here, S/N, which means signal of the luminescence of the positive samples over that of a negative sample.
After thoroughly washing, 5 μL 200 μM coelenterazine (Glpbio) were then added in the suspended magnetic beads for the measurement of relative light unit using a Synergy TM H1 multi-mode microplate reader.
Analytica Chimica Acta (2022): 340633. PMID: 36464447 IF: 6.9111

Product Documents

Quality Control & SDS

View current batch:

Purity: >98.00%

Protocol

Cell experiment [1]:
Cell lines	RAW 264.7 cells
Preparation Method	5 × 10⁴ cRAW 264.7 cells were added to wells of a 96-well black plate in triplicate, cell counts were performed, and native coelenterazine was added to the sample (final coelenterazine concentration 0-100 μmol/l). Plates were imaged using an IVIS low-light imaging system
Reaction Conditions	final coelenterazine concentration 0-100 μmol/l,60min
Applications	RAW 264.7 chemiluminescent signal intensity positively correlates with coelenterazine concentration in culture. The production of chemiluminescence by RAW 264.7 cells in the absence of stimulation is consistent with the detection of superoxide anion production during oxidative phosphorylation.
Animal experiment [2]:
Animal models	10-26 weeks thirty-six mice (16 females, 20 males)
Preparation Method	450 nl of virus (three sites) were injected into the cerebral cortex of mice, followed by coelenterazine injections at 28-day intervals (minimum = 26 days, maximum time = 74 days). Coelenterazine (dissolved in DMSO) and diluted to a concentration of 2.43 mM in sterile water (1 mg/ml) for cortical injection through a 34-gauge needle for imaging and electrophysiological recordings.
Dosage form	0.2µl, 0.4µl and 1µl,the injection rate was 1.25 μL/min
Applications	Coelenterazine strongly boosted activity in LMO3-expressing mice, photon production was proportional to emission rate, and maximal doses of coelenterazine moderately increased neural activity in naive mice and GLuc cohorts, which itself can be produced at high concentrations Nonspecific excitatory effects.
References: [1]. Bronsart L, Nguyen L, Habtezion A, Contag C. Reactive Oxygen Species Imaging in a Mouse Model of Inflammatory Bowel Disease. Mol Imaging Biol. 2016 Aug;18(4):473-8. [2]. Gomez-Ramirez M, More AI,et al. The BioLuminescent-OptoGenetic in vivo response to coelenterazine is proportional, sensitive, and specific in neocortex. J Neurosci Res. 2020 Mar;98(3):471-480.

Coelenterazine ist ein Substrat verschiedener Luciferasen wie Renilla-Luciferase (Rluc) und Gaussia-Luciferase (Gluc) und ist auch ein Kofaktor von Aequorin. Es wird häufig verwendet und kann zur Detektion der Kalziumkonzentration in lebenden Zellen, bei Genreporter-Assays, BRET (Biolumineszenz-Resonanzenergietransfer)-Studien, ELISA, HTS und zur Chemilumineszenzdetektion von ROS-Niveaus in Geweben oder Zellen eingesetzt werden[1].

In vitro ermöglichte Coelenterazin die dynamische Überwachung des oxidativen Ausbruchs der RAW 264.7-Zellen[2]. Die Chemilumineszenz von Coelenterazin hing nicht von der Aktivität der zellulären Myeloperoxidase ab. Es ist eine sehr empfindliche und spezifische Chemilumineszenzsonde für Superoxidanionen[3]. Coelenterazin ist eine zuverlässigere Sonde, obwohl es lagerungsabhängiger hoher Hintergrundchemilumineszenz ausgesetzt ist, jedoch keine Prereduktion erfordert[4].

In vivo gab es frühe, präklinische Veränderungen in der Lokalisation und dem Ausmaß der Coelenterazin-Chemilumineszenzherde[2]. Handelsübliches Coelenterazin (löslich in DMSO) ist ein empfindliches Substrat für die in vivo Biolumineszenzbildgebung von Coelenterazin-Luciferase[5].

References:
[1]Jiang T, Du L, Li M. Lighting up bioluminescence with coelenterazine: strategies and applications. Photochem Photobiol Sci. 2016 Apr;15(4):466-80.
[2] Bronsart L, Nguyen L, et al. Reactive Oxygen Species Imaging in a Mouse Model of Inflammatory Bowel Disease. Mol Imaging Biol. 2016 Aug;18(4):473-8.
[3]Teranishi K. Non-invasive and accurate readout of superoxide anion in biological systems by near-infrared light. Anal Chim Acta. 2021 Sep 22;1179:338827.
[4] Kervinen M, Pätsi J, Finel M, Hassinen IE. Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes. Anal Biochem. 2004 Jan 1;324(1):45-51.
[5]Lucas M, Solano F. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils. Anal Biochem. 1992 Nov 1;206(2):273-7.

Cas No.	55779-48-1	SDF
Überlieferungen	Luciferin (Oplophorus), Preluciferin (Watasenia)
Chemical Name	8-benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one
Canonical SMILES	OC1=CC=C(CC(C2=O)=NC3=C(NC(C4=CC=C(O)C=C4)=CN32)CC5=CC=CC=C5)C=C1
Formula	C₂₆H₂₁N₃O₃	M.Wt	423.46
Löslichkeit	Ethanol : 2 mg/mL (4.72 mM; Need ultrasonic; DMSO can inactivate Coelenterazine's activity)	Storage	-20°C, protect from light, stored under nitrogen,unstable in solution, ready to use.
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
		1 mg	5 mg	10 mg
	1 mM	2.3615 mL	11.8075 mL	23.615 mL
	5 mM	0.4723 mL	2.3615 mL	4.723 mL
	10 mM	0.2361 mL	1.1807 mL	2.3615 mL

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Average Rating: 5 ★★★★★ (Based on Reviews and 28 reference(s) in Google Scholar.)

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Coelenterazine (Synonyms: Luciferin (Oplophorus), Preluciferin (Watasenia))

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Product has been cited by 4 publications

Related Biological Data

Related Biological Data

Related Biological Data

Related Biological Data

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

Molaritätsrechner

Verdünnung-Rechner

Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

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