D-Luciferin (potassium salt) |
Katalog-Nr.GC43496 |
D-Luciferin (D-(-)-Luciferin) Kalium ist das Substrat von Luciferasen, die die Produktion von Licht in biolumineszenten Insekten katalysieren.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 115144-35-9
Sample solution is provided at 25 µL, 10mM.
D-luciferin is the natural substrate of firefly luciferase. In the presence of magnesium ions, luciferase catalyzes the reaction of luciferin with ATP, which is then oxidized to form a dioxetane structure that emits yellow-green light [1]. When the substrate luciferin is in excess, the 560 nm chemiluminescence generated by the Luciferin-luciferase luminescent reaction reaches its peak within a few seconds, and the light output is proportional to the luciferase concentration. Chemiluminescent techniques are virtually background-free, making the luciferase reporter an ideal tool for detecting low-level gene expression. 0.02 pg of luciferase can be reliably measured in a standard fluorescence counter. D-luciferin is a commonly used reporter gene for ATP detection, cell viability assay, reporter gene detection, active molecular screening and bacterial counting. D-luciferin is widely used in live animal imaging. Cells expressing the luciferase gene were transplanted into research animals and injected with D-luciferin to be able to detect changes in brightness by bioluminescence imaging (BLI)[2].
D-Luciferin has three product forms, D-Luciferin (D-Luciferin, free acid), D-Luciferin potassium salt (D-Luciferin, potassium salt) and D-Luciferin sodium salt (D-Luciferin, sodium salt ). The potassium and sodium salt forms of D-fluorescein are the most versatile because they are both readily soluble in water. Potassium salt is also the main form used in live animal testing.
References:
[1]. Giuseppe Meroni, et al. D-Luciferin, derivatives and analogues: synthesis and in vitro/in vivo luciferase-catalyzed bioluminescent activity. ARKIVOC 2009 (i) 265-288.
[2]. Sangyub Kim, et al. Optimizing live-animal bioluminescence imaging prediction of tumor burden in human prostate cancer xenograft models in SCID-NSG mice.2019 Jun;79(9):949-960. doi: 10.1002/pros.23802. Epub 2019 Apr 8.
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