Fluorescein Tyramide |
| Katalog-Nr.GC50456 |
Fluorescein-Tyramid ist ein grÜn fluoreszierendes Reagenz (Λabs: 494 nm; Λem: 517 nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 210236-90-1
Sample solution is provided at 25 µL, 10mM.
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Green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
Karkmann et al (1999) Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry. J.Immunol.Methods 230 113 PMID:10594358 |Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID:10489619 |Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID:9603790 |van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID:9071319 |Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID:2558138
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