HOE 32021 |
Katalog-Nr.GC36238 |
Hoechst-FÄrbemittel sind Teil einer Familie blau fluoreszierender Farbstoffe, die zum FÄrben von DNA verwendet werden.
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Cas No.: 23623-06-5
Sample solution is provided at 25 µL, 10mM.
HOE 32021 is a Hoechst series of dyes that are commonly used for nuclear labeling staining of living cells. Hoechst dyes can be used to monitor cell viability by tracking changes in their emission spectra. As slightly groove-binding DNA stains with AT selectivity, Hoechst dyes are able to bind to all nucleic acids, but they show greater fluorescence enhancement for AT-rich double-stranded DNA strands compared to GC-rich strands [1]. This property has been used to identify Q bands in chromosomes, which are AT base pair-rich regions that fluoresce brightly when stained with quinacrine dye [2]. The fluorescence intensity of Hoechst dye increases as the pH of the solution increases.
References:
[1]. Karen E. Selph. Enumeration of marine microbial organisms by flow cytometry using near-UV excitation of Hoechst 34580-stained DNA.
[2]. Jonas Bucevičius, Gražvydas Lukinavičius, Rūta Gerasimaitė. The Use of Hoechst Dyes for DNA Staining and Beyond. Chemosensors 2018, 6(2), 18. Doi:org/10.3390/chemosensors6020018.
Average Rating: 5
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