Hydroxy-γ-sanshool |
| Katalog-Nr.GC36281 |
Hydroxy-γ-sanshool is a natural alkaloid extracted from Zanthoxylum plants and belongs to the class of unsaturated fatty acid amide compounds.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 78886-66-5
Sample solution is provided at 25 µL, 10mM.
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Hydroxy-γ-sanshool is a natural alkaloid extracted from Zanthoxylum plants and belongs to the class of unsaturated fatty acid amide compounds[1]. Hydroxy-γ-sanshool has multiple physiological activities such as neuroactivation, analgesia, anti-inflammatory, and antioxidant[2, 3]. Hydroxy-γ-sanshool can activate TRPV1 channels in human sensory neurons and trigger calcium ion influx[4].
In vitro, treatment of human colon adenocarcinoma HCT-116 cells with Hydroxy-γ-sanshool (0-130µM) for 24-48h inhibited cell growth, induced cell morphological distortion, induced cell cycle arrest in the G1 phase, caused programmed cell death, reduced the mRNA and protein levels of Cyclin D1, CDK4, and PCNA, and increased the mRNA and protein levels of P21, P53, Fas, and Caspase 8[5].
In vivo, oral administration of Hydroxy-γ-sanshool (2.5mg/kg) to mice induced by dextran sulfate sodium (DSS)-induced oxidative stress increased the hepatic antioxidant capacity (T-AOC) and the levels of colonic GSH-Px and SOD[6].
References:
[1] Lu I C, Hu P Y, Lin C H, et al. Alkamides in Zanthoxylum Species: Phytochemical Profiles and Local Anesthetic Activities[J]. International Journal of Molecular Sciences, 2024, 25(22): 12228.
[2] Yan H, Zhou F, Liu Y, et al. Zanthoxylum bungeanum Maxim: A Review of Its Phytochemistry, Pharmacology, and Pharmacokinetics[J]. Natural Product Communications, 2023, 18(10): 1934578X231209153.
[3] Li R L, Zhang Q, Liu J, et al. Hydroxy‐α‐sanshool Possesses Protective Potentials on H2O2‐Stimulated PC12 Cells by Suppression of Oxidative Stress‐Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway[J]. Oxidative Medicine and Cellular Longevity, 2020, 2020(1): 3481758.
[4] Luo J, Ke J, Hou X, et al. Composition, structure and flavor mechanism of numbing substances in Chinese prickly ash in the genus Zanthoxylum: A review[J]. Food Chemistry, 2022, 373: 131454.
[5] Zhaojun C, Lulin T, Xin F, et al. Hydroxy-γ-sanshool from Zanthoxylum bungeanum (prickly ash) induces apoptosis of human colorectal cancer cell by activating P53 and Caspase 8[J]. Frontiers in Nutrition, 2022, 9: 914638.
[6] Chen Z, Tan L, Li L, et al. Sanshools from Zanthoxylum genus and their antioxidant properties in vitro and in vivo[J]. Frontiers in Sustainable Food Systems, 2024, 8: 1256568.
| Cell experiment [1]: | |
Cell lines | HCT-116 cells |
Preparation Method | Cells were exposed to various concentrations (0, 50, 90, or 130µM) of Hydroxy-γ-sanshool (HRS) for 24 or 48h, with assessment of apoptosis and cell cycle arrest as well as regulatory gene and protein expression associated with apoptosis and the cell cycle. |
Reaction Conditions | 0, 50, 90, 130µM; 24, 48h |
Applications | Hydroxy-γ-sanshool inhibited cell growth, induced morphological distortion, and arrested the cell cycle at G1 phase, and also caused programmed cell death in a dose-dependent manner. Hydroxy-γ-sanshool significantly inhibited mRNA and protein levels of Cyclin D1, CDK4, PCNA, and increased mRNA and protein levels of P21, P53, Fas, and Caspase 8. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | Mice were randomly divided into the following six groups: the normal control group, Dextran sulfate sodium (DSS)-model group, DSS+Hydroxy-α-sanshool (HAS) group, DSS+Hydroxy-γ-sanshool (HBS) group, DSS+Hydroxy-γ-sanshool (HRS) group, DSS+γ-sanshool (RS) group. The intragastric dose of sanshool was 2.5mg/kg. After mice were termination, the liver and colon were collected at −80°C. |
Dosage form | 2.5mg/kg; p.o. |
Applications | After intragastric administration of sanshool, HRS increased the the level of hepatic T-AOC and colon GSH-Px and SOD. Hydroxy-γ-sanshool exhibited efficacy in mitigating DSS-induced oxidative stress in the colon of mice. |
References: | |
| Cas No. | 78886-66-5 | SDF | |
| Canonical SMILES | C/C=C/C=C/C=C\CC/C=C/C=C/C(NCC(C)(C)O)=O | ||
| Formula | C18H27NO2 | M.Wt | 289.41 |
| Löslichkeit | Soluble in DMSO | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4553 mL | 17.2765 mL | 34.5531 mL |
| 5 mM | 0.6911 mL | 3.4553 mL | 6.9106 mL |
| 10 mM | 0.3455 mL | 1.7277 mL | 3.4553 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)
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