Hydroxy naphthol blue disodium |
| Katalog-Nr.GC67465 |
Hydroxynaphtholblaudinatrium, ein Azofarbstoff, ist ein Metallindikator für Calcium und ein kolorimetrisches Reagenz für Erdalkalimetallionen ( Λmax \u003d 650 nm). Im pH-Bereich zwischen 12 und 13 ist die Lösung des Indikators in Gegenwart von Calciumionen rötlich-rosa und in Gegenwart von Dinatrium-EDTA tiefblau.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 165660-27-5
Sample solution is provided at 25 µL, 10mM.
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Hydroxy naphthol blue disodium, an azo dye, is a metal indicator for calcium and a colorimetric reagent for alkaline earth metal ions ( Λmax = 650 nm). In the pH range between 12 and 13, the solution of the indicator is reddish pink in the presence of calcium ion and to deep blue in the presence of disodium EDTA[1].
[1]. Motoki Goto, et al. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques. 2009 Mar;46(3):167-72.
| Cas No. | 165660-27-5 | SDF | |
| Formula | C20H12N2Na2O11S3 | M.Wt | 598.49 |
| Löslichkeit | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.6709 mL | 8.3544 mL | 16.7087 mL |
| 5 mM | 0.3342 mL | 1.6709 mL | 3.3417 mL |
| 10 mM | 0.1671 mL | 0.8354 mL | 1.6709 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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