IRBP (1-20), human |
| Katalog-Nr.GC30558 |
IRBP (1-20), human ist das 1-20 Aminosäurefragment des optischen Interrezeptor-Retinoid-bindenden Proteins (IRBP).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 298202-25-2
Sample solution is provided at 25 µL, 10mM.
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IRBP (1-20), human ist das 1-20 Aminosäurefragment des optischen Interrezeptor-Retinoid-bindenden Proteins (IRBP). IRBP ist ein 140 kDa Glykoprotein, das in der Photorezeptormatrix zwischen der neuronalen Retina und dem retinalen Pigmentepithel gefunden wird. Humane IRBP-Peptide 1-20 enthalten ein Haupt-Epitop des H-2b-Haplotyps. Die Immunität gegen IRBP (1-20) induziert eine T-Zell-vermittelte experimentelle autoimmune Uveoretinitis (EAU) [1,2].
Weibliche C57BL/6J-Mäuse, die durch IRBP (1-20), human induziert wurden, zeigten erfolgreich das EAU-Phänotyp [3,5]. Die Konsequenzen eines IRBP-Mangels auf das T-Zell-Repertoire wurden ebenfalls untersucht. IRBP+/+, IRBP+/- und IRBP-/- Mäuse auf dem C57BL/6-Hintergrund wurden mit IRBP oder einem pathogenen Epitop, dem IRBP (1-20), human Peptid im Adjuvans, immunisiert und auf Krankheitschwere und immunologische Reaktionen hin bewertet. C57BL/6 IRBP-/- Mäuse waren vollständig resistent gegen EAU und EAP und zeigten verstärkte immunologische Reaktionen auf IRBP und sein pathogenes Peptid 1-20 im Vergleich zu ihren IRBP+/+ Gegenstücken. IRBP-/- Mäuse zeigten eine veränderte IRBP-Epitoperkennung [4].
References:
[1]. Avichezer D, Chan CC,et,al. Residues 1-20 of IRBP and whole IRBP elicit different uveitogenic and immunological responses in interferon gamma deficient mice. Exp Eye Res. 2000 Aug;71(2):111-8. doi: 10.1006/exer.2000.0860. PMID: 10930316.
[2]. Avichezer D, Silver PB,et,al. Identification of a new epitope of human IRBP that induces autoimmune uveoretinitis in mice of the H-2b haplotype. Invest Ophthalmol Vis Sci. 2000 Jan;41(1):127-31. PMID: 10634611.
[3]. Meng X, Fang S, et,al. Preventive effect of chrysin on experimental autoimmune uveitis triggered by injection of human IRBP peptide 1-20 in mice. Cell Mol Immunol. 2017 Aug;14(8):702-711. doi: 10.1038/cmi.2015.107. Epub 2016 Mar 21. PMID: 26996065; PMCID: PMC5549600.
[4]. Avichezer D, Liou GI, et,al. Interphotoreceptor retinoid-binding protein (IRBP)-deficient C57BL/6 mice have enhanced immunological and immunopathogenic responses to IRBP and an altered recognition of IRBP epitopes. J Autoimmun. 2003 Nov;21(3):185-94. doi: 10.1016/j.jaut.2003.08.004. PMID: 14599843.
[5]. Tajiri N, Kato T, et,al. The protective function of invariant natural killer T cells in the relapse of experimental autoimmune uveoretinitis. Exp Eye Res. 2021 Feb;203:108406. doi: 10.1016/j.exer.2020.108406. Epub 2020 Dec 19. PMID: 33347870.
| Animal experiment [1]: | |
Animal models | Female C57BL/6J mice (6-8 weeks of age) |
Preparation Method | Each C57BL/6J mouse was immunized subcutaneously in one rear footpad and the tail head, and rump (total of 200 μl) with an emulsion containing 300 μg of IRBP (1-20), human in phosphate-buffered saline (PBS) and 300 μg of Mycobacterium tuberculosis H37Ra in complete Freund s adjuvant. |
Dosage form | 300 μg IRBP (1-20), human |
Applications | Female C57BL/6J mice induced by IRBP (1-20), human successfully showed eau phenotype. |
References: | |
| Cas No. | 298202-25-2 | SDF | |
| Canonical SMILES | Gly-Pro-Thr-His-Leu-Phe-Gln-Pro-Ser-Leu-Val-Leu-Asp-Met-Ala-Lys-Val-Leu-Leu-Asp | ||
| Formula | C101H164N24O28S | M.Wt | 2194.59 |
| Löslichkeit | Soluble in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.4557 mL | 2.2783 mL | 4.5567 mL |
| 5 mM | 0.0911 mL | 0.4557 mL | 0.9113 mL |
| 10 mM | 0.0456 mL | 0.2278 mL | 0.4557 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)
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