PtdIns-(1-arachidonoyl, 2-arachidonoyl-d8) (ammonium salt)

PtdIns-(1-arachidonoyl, 2-arachidonoyl-d₈) is used as an internal standard for the quantification of PtdIns-(1-arachidonoyl, 2-arachidonoyl) by stable isotope dilution MS. The accuracy of the sample weight in this vial is between 5% over and 2% under the amount shown on the vial. If better precision is required, the deuterated standard should be quantitated against a more precisely weighed unlabeled standard by constructing a standard curve of peak intensity ratios (deuterated versus unlabeled). The phosphatidylinositols (PtdIns) represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.^1,2 PtdIns-(1-arachidonoyl, 2-arachidonoyl-d₈) (sodium salt) is a synthetic analog of natural PtdIns featuring deuterated C₂₀:4 fatty acids at the sn-2 position. This synthetic standard compound contains the same inositol and diacyl glycerol (DAG) stereochemistry as that of the natural compound.

1.Lapetina, E.G., Billah, M.M., and Cuatrecasas, P.The phosphatidylinositol cycle and the regulation of arachidonic acid productionNature292367-369(1981) 2.Pike, L.J., and Casey, L.Localization and turnover of phosphatidylinositol 4,5-bisphosphate in caveolin-enriched membrane domainsThe Journal of Biological Chemisty27126453-26456(1996)