PA Janelia Fluor 646, SE (Synonyms: PA-JF646-NHS) |
| Katalog-Nr.GC50362 |
PA Janelia Fluor 646, SE (PA-JF646-NHS), ein photoaktivierbarer Fluoreszenzfarbstoff, ist ein NHS-Ester zur Kopplung an primäre Amingruppen.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2093120-32-0
Sample solution is provided at 25 µL, 10mM.
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Photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor® 549, SE to perform two-colour sptPALM in live cells with activation at 405nm. Cell permeable. Excitation maximum = 649 - 651 nm; emission maximum = 663 - 665 nm. Upon protein conjugation the efficiencies of photoconversion improve substantially. Low background staining. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID:27776112 |Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID:27484239
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
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