Rose Bengal (Synonyms: Acid Red 94, Japan Red 105-1, Red No. 105-1) |
| Katalog-Nr.GC15691 |
Rose Bengal, ein synthetisches Fluorescein-Derivat, ist ein purpurroter Farbstoff, dessen Hauptbestandteil 4,5,6,7-Tetrachlor-2,4,5,7-Tetraiod-Fluorescein ist.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 632-69-9
Sample solution is provided at 25 µL, 10mM.
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Rose Bengal is a potent inhibitor of VGlut and vesicular monoamine transporter (VMAT), with Ki values of 19nM and 64nM, respectively [1]. Rose Bengal can be used as a dye, photosensitizer[2], and can also be used in the research of colon cancer, breast cancer, and ovarian cancer[3][4][5].
Rose Bengal (100-800μM; 4 days) dose-dependently inhibited the growth of UWB and BRCA1 ovarian cancer cells[3]. Rose Bengal (160μM; 60s) significantly enhanced ultrasound-induced injury in sarcoma cells in vitro, with 160μM Rose Bengal increasing the rate of ultrasound-induced cell injury by 2 to 3 times[4]. After treating MCF-7 with Rose Bengal (200-300μM; 24-48h) for 24h, MCF-7 cells undergo morphological changes, including volume reduction and rounding, until the nucleus constitutes the majority of the cell volume[5]. Rose Bengal (25-1000μM; 24h)-treated colon cancer cells expressed distinct hallmarks of immunogenic cell death (ICD), including enhanced expression of calreticulin and heat-shock protein 90 on the cell surface, a decrease in intracellular ATP, and the release of HMGB1[6].
Rose Bengal (300μM; 24h)-treated colon cancer served as a vaccine against subsequent challenge with the same CT26 colon cancer tumor cells, and vaccination with in vitro Rose Bengal-treated cells resulted in slower tumor growth following inoculation with colon cancer cells[6].Intralesional injection of Rose Bengal (50μL 10% Rose Bengal) induces systemic tumor-specific immune responses in mouse models of melanoma and breast cancer[7].
References:
[1]. Ogita K, Hirata K, Bole D G, et al. Inhibition of vesicular glutamate storage and exocytotic release by Rose Bengal[J]. Journal of neurochemistry, 2001, 77(1): 34-42.
[2]. Watts L T, Zheng W, Garling R J, et al. Rose Bengal photothrombosis by confocal optical imaging in vivo: a model of single vessel stroke[J]. Journal of Visualized Experiments: JoVE, 2015 (100).
[3]. Koevary S B. Selective toxicity of rose bengal to ovarian cancer cells in vitro[J]. International journal of physiology, pathophysiology and pharmacology, 2012, 4(2): 99.
[4]. Umemura S, Yumita N, Umemura K, et al. Sonodynamically induced effect of rose bengal on isolated sarcoma 180 cells[J]. Cancer chemotherapy and pharmacology, 1999, 43: 389-393.
[5]. Mousavi S H, Tavakkol-Afshari J, Brook A, et al. Direct toxicity of Rose Bengal in MCF-7 cell line: role of apoptosis[J]. Food and chemical toxicology, 2009, 47(4): 855-859.
[6]. Qin J, Kunda N, Qiao G, et al. Colon cancer cell treatment with rose bengal generates a protective immune response via immunogenic cell death[J]. Cell death & disease, 2017, 8(2): e2584-e2584.
[7]. Toomey P, Kodumudi K, Weber A, et al. Intralesional injection of rose bengal induces a systemic tumor-specific immune response in murine models of melanoma and breast cancer[J]. PloS one, 2013, 8(7): e68561.
| Cell experiment [1]: | |
Cell lines | UWB and BRCA1 ovarian cancer cells |
Preparation Method | UWB and BRCA1 ovarian cancer cells were incubated with 100-800μM Rose Bengal for four days. |
Reaction Conditions | 100-800μM; 4 days |
Applications | Rose Bengal dose-dependently inhibited the growth of UWB and BRCA1 ovarian cancer cells. |
| Animal experiment [2]: | |
Animal models | Colon cancer tumor modle |
Preparation Method | CT26 cells were treated in vitro with Rose Bengal (300μM) for 24h and the cells were harvested and washed with PBS. A total of 1×106 Rose Bengal-treated cells in 100μl PBS were injected subcutaneously into the upper right flank of each mouse, on days −14 and −7, whereas control mice received 100μl injections of PBS. One week after the second vaccination (day 0), 1×106 untreated CT26 cells and 1×106 4T1 murine breast cancer cells were inoculated into the lower right flank and upper left flank, respectively. Mice were killed 2 weeks after the challenge injection and the tumors were excised in their entirety. |
Dosage form | 300μM; 24h |
Applications | Rose Bengal-treated tumors served as a vaccine against subsequent challenge with the same CT26 colon cancer tumor cells, and vaccination with in vitro Rose Bengal-treated cells resulted in slower tumor growth following inoculation with colon cancer cells. |
References: | |
| Cas No. | 632-69-9 | SDF | |
| Überlieferungen | Acid Red 94, Japan Red 105-1, Red No. 105-1 | ||
| Chemical Name | sodium 2,3,4,5-tetrachloro-6-(2,4,5,7-tetraiodo-6-oxido-3-oxo-3H-xanthen-9-yl)benzoate | ||
| Canonical SMILES | IC1=C(C(C(C(C(C([O-])=O)=C2Cl)=C(C(Cl)=C2Cl)Cl)=C3C=C4I)=CC(I)=C1[O-])OC3=C(C4=O)I.[Na+].[Na+] | ||
| Formula | C20H2Cl4I4Na2O5 | M.Wt | 1017.64 |
| Löslichkeit | <101.76mg/ml in Water; <50.88mg/ml in DMSO | Storage | Store at RT, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.9827 mL | 4.9133 mL | 9.8267 mL |
| 5 mM | 0.1965 mL | 0.9827 mL | 1.9653 mL |
| 10 mM | 0.0983 mL | 0.4913 mL | 0.9827 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)
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