TC-T 6000 |
| Katalog-Nr.GC50098 |
TC-T 6000 ist ein potenter und selektiver Inhibitor des menschlichen ENT4 (Equilibrative Nukleosidtransporter 4) mit einer IC50 von 74,4 nM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 949467-71-4
Sample solution is provided at 25 µL, 10mM.
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TC-T 6000, a Dipyridamole analog, is a potent and selective human ENT4 (Balanced nucleoside transporter 4) inhibitor with an IC50 of 74.4nM. Making it about 38 times more potent than Dipyridamole [1].
TC-T 6000(10μM; 24h) shows no cytotoxicity against PK15/hENT4 cells.
References:
[1]. Wang C, Lin W, et,al. Dipyridamole analogs as pharmacological inhibitors of equilibrative nucleoside transporters. Identification of novel potent and selective inhibitors of the adenosine transporter function of human equilibrative nucleoside transporter 4 (hENT4). Biochem Pharmacol. 2013 Dec 1;86(11):1531-40. doi: 10.1016/j.bcp.2013.08.063. Epub 2013 Sep 7. PMID: 24021350; PMCID: PMC3866046.
| Cell experiment [1]: | |
Cell lines | PK15/hENT cells (PK15 cells stably expressing hENT4) |
Preparation Method | Cells were seeded at 5×103 cells were seeded in 96-well plates, treated with 10μM test TC-T 6000 and incubated at 37 ℃ in phenol-free MEM for 24h. |
Reaction Conditions | 10μM; 24h |
Applications | TC-T 6000 shows no cytotoxicity against PK15/hENT4 cells. |
| Transporter inhibition assays [1]: | |
Preparation Method | Transport activity of cells transiently transfected with hENT4 was examined with [3H] Adenosine. K15/hENT4 cells were incubated with transport buffer containing TC-T 6000 at pH 6.0 for 15min before addition of 0.2μM [3H] Adenosine, and then further incubation for 2min. Uptake was terminated by rapidly aspirating the incubation mixture and washing cells three times with ice-cold PBS. The cells were solubilized overnight in 400μl of 5% Triton X-100 and 200μl of cell lysate was counted using a scintillation counter. Protein concentration in the cell lysate was determined using the BCA protein assay kit. The concentrations of test compounds that caused 50% inhibition of [3H] Adenosine uptake (IC50) were calculated using a nonlinear fitting method in the Prism 4 program. |
Reaction Conditions | 10μM; 15min |
Applications | TC-T 6000 is a potent and selective human ENT4 (Balanced nucleoside transporter 4) inhibitor with an IC50 of 74.4nM. |
References: | |
| Cas No. | 949467-71-4 | SDF | |
| Canonical SMILES | CC(C)CN(CC(C)C)C2=NC(NCCO)=NC1=C(N(CC(C)C)CC(C)C)N=C(NCCO)N=C12 | ||
| Formula | C26H48N8O2 | M.Wt | 504.71 |
| Löslichkeit | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.9813 mL | 9.9067 mL | 19.8134 mL |
| 5 mM | 0.3963 mL | 1.9813 mL | 3.9627 mL |
| 10 mM | 0.1981 mL | 0.9907 mL | 1.9813 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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