DU717 |
| Catalog No.GC32647 |
DU-717 is an antihypertensive agent.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 59943-31-6
Sample solution is provided at 25 µL, 10mM.
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DU-717 is an antihypertensive agent.
A gas chromatographic method has been developed which enables accurate determination of a new antihypertensive agent, DU-717, in plasma. Accurate determinations are possible over a concentration range from 10 to 150 ng/mL of DU-717 in plasma at a relative standard deviation of 6.2%. The minimum detectable concentration is 1 ng/mL. Plasma levels of DU-717 in spontaneously hypertensive and normotensive rats following single oral administrations (10 mg/kg) have also been determined[1].
[1]. Yamaguchi T, et al. Determination of 7-chloro-3-(4-methyl-1-piperazinyl)-4h-1,2,4-benzothiadiazine-1,1-dioxide (DU-717) in plasma using electron-capture gas chromatography. J Chromatogr. 1978 Oct 11;160(1):181-90. [2]. R Horie. rats (ALR) and normotensive atherogenic rats (NAR).
Animal experiment: | Rats[2] DU-717 (100mg/kg per day, p.o.)-treated SHRSP, SHRSP fed on a HFC diet (including 20% suet, 5% cholesterol and 2% cholic acid) with 1% NaCl in the drinking water, ALR and NAR, 15 rats in each group, are used for EEG recording. Rats, anesthetized with intraperitoneal injection of pentobarbital (30mg/kg), are placed in a stereotaxic frame for exposing the skull, and 6 small pores of 1mm in diameter are made by a dental drill at frontal, parietal, and occipital areas on both sides. Then, stainless steel electrodes (220μ in diameter), insulated except a small loop which is formed at the tip in order to avoid any injury to the dura and the cortex, are inserted through the pores of the skull and settled gently on the dura. Electrodes on both sides are located 2mm apart from mid-sagittal line, and frontal (F), parietal (P), occipital (O) electrodes are located 5mm, 2.5 mm, and 0mm anterior to the interauricular line, respectively. The indifferent electrode (I) is placed on the mid-sagittal line, 4mm anterior to the bregma. All electrodes are connected with insulated copper wires to a miniature receptacle, and the whole assembly is embedded in dental cement. Two weeks after the operation, EEG is repeatedly recorded for several months. When EEG is recorded, rats are in a clear plastic cage (30×40×40cm) and could move freely. EEG is recorded by a ME-920, connected to an autoanalyzer MAF-5, and a photic stimulator is MS-2PS. Monopolar (I-F, I-P, I-O), and bipolar (F-P, P-O, O-F) leading are recorded. EEG is recorded during wakefulness and drowsiness. The recording calibration is 3cm/sec and 100μV/4mm. |
References: [1]. Yamaguchi T, et al. Determination of 7-chloro-3-(4-methyl-1-piperazinyl)-4h-1,2,4-benzothiadiazine-1,1-dioxide (DU-717) in plasma using electron-capture gas chromatography. J Chromatogr. 1978 Oct 11;160(1):181-90. | |
| Cas No. | 59943-31-6 | SDF | |
| Canonical SMILES | CN1CCN(C2=NC3=CC=C(Cl)C=C3S(N2)(=O)=O)CC1 | ||
| Formula | C12H15ClN4O2S | M.Wt | 314.79 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.1767 mL | 15.8836 mL | 31.7672 mL |
| 5 mM | 0.6353 mL | 3.1767 mL | 6.3534 mL |
| 10 mM | 0.3177 mL | 1.5884 mL | 3.1767 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)
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