eGFP mRNA with N1-Me-pUTP (5’CAP) |
| Catalog No.GM10004 |
eGFP mRNA with N1-Me-pUTP (5'CAP) is a labeled green fluorescent protein mRNA produced by in vitro transcription, has a Cap 1 cap structure and a poly(A) tail, and contains N1-Me-pUTP modification.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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eGFP is a commonly used reporter gene. eGFP mRNA directly expresses protein in the cytoplasm without relying on activation. The protein expression speed is faster than transfected DNA, and there is no risk of genome integration. Protein expression is directly related to the amount of mRNA. Cells transfected with eGFP mRNA can express strong and bright green fluorescent protein, and the excitation/emission wavelengths are 488 nm/509 nm, respectively. eGFP mRNA can be used as a standard to detect the transfection efficiency of different transfection reagents, and can also be used as a control to study the transfection and expression of fluorescent proteins in mammalian cells.
By simulating the mRNA processing process in eukaryotes, the product has a Cap 1 cap structure at the 5' end and a poly(A) tail at the 3' end, which increases the stability and translation efficiency of mRNA[1]. N1-methyl-pseudouridine (1-methylpseudouridine, m1ψ) is a methyl modification of naturally occurring pseudouridine, formed by N1-specific pseudouridine A that exists in archaea and eukaryotes It is catalyzed by the base transferase Nepl[2]. N1-methyl-pseudouridine is a substitute for uridine (U), which can effectively enhance RNA stability while reducing anti-RNA immune response [3].
References:
[1]. Jemielity J, Fowler T, Zuberek J, et al. Novel "anti-reverse" cap analogs with superior translational properties. RNA. 2003;9(9):1108-1122
[2]. Callum J C Parr, et al.N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells. 2020 Apr 6;48(6):e35. doi: 10.1093/nar/gkaa070.
[3]. Pedro Morais, Hironori Adachi, Yi-Tao Yu.The Critical Contribution of Pseudouridine to mRNA COVID-19 Vaccines. 2021 Nov 4;9:789427. doi: 10.3389/fcell.2021.789427.
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| Buffer | Storage | -40°C or below | |
| General tips | Please dissolve it on ice and be careful to prevent RNase contamination and degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For first use, gently centrifuge and divide into portions for individual use. Use RNase-free reagents and consumables, use appropriate RNase-free techniques, and do not add to the serum-containing medium until mixed with the transfection reagent. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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