(±)12-HETE-d8 (Synonyms: (±)12-Hydroxyeicosatetraenoic Acid-d8)

(±)12-HETE-d₈ is intended for use as an internal standard for the quantification of 12-HETE by GC- or LC-MS. (±)12-HETE is formed via non-enzymatic oxidation of arachidonic acid .^1,2 12(S)- and 12(R)-HETE are formed by 12(S)- and 12(R)-lipoxygenase-mediated oxidation of arachidonic acid, respectively.^3,4 12(R)-HETE can also be formed by oxidation of arachidonic acid mediated by cytochrome P450s (CYP450s).⁵

1.Powell, W.S., and Rokach, J.Biosynthesis, biological effects, and receptors of hydroxyeicosatetraenoic acids (HETEs) and oxoeicosatetraenoic acids (oxo-ETEs) derived from arachidonic acidBiochim. Biophys. Acta1851(4)340-355(2014) 2.Derogis, P.B.M.C., Chaves-Filho, A.B., and Miyamoto, S.Characterization of hydroxy and hydroperoxy polyunsaturated fatty acids by mass spectrometryBioactive lipids in health and disease21-38(2019) 3.KÜhn, H., Banthiya, S., and van Leyen, K.Mammalian lipoxygenases and their biological relevanceBiochim. Biophys. Acta1851(4)308-330(2015) 4.BÜrger, F., Krieg, P., Marks, F., et al.Positional- and stereo-selectivity of fatty acid oxygenation catalysed by mouse (12S)-lipoxygenase isoenzymesBiochem J.348(Pt 2)329-335(2000) 5.Powell, W.S., and Rokach, J.Biosynthesis, biological effects, and receptors of hydroxyeicosatetraenoic acids (HETEs) and oxoeicosatetraenoic acids (oxo-ETEs) derived from arachidonic acidBiochim. Biophys. Acta1851(4)340-355(2015)