4-Nitrophenyl Phosphate (sodium salt hydrate) (Synonyms: p-Nitrophenyl Phosphate, para-Nitrophenyl Phosphate, 4-NPP, pNPP) |
Catalog No.GC49158 |
L'hexahydrate disodique de phosphate de 4-nitrophényle (phosphate de p-nitrophényle) est largement utilisé comme substrat de type phosphotyrosine À petite molécule dans les dosages d'activité des protéines tyrosine phosphatases.
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Cas No.: 333338-18-4
Sample solution is provided at 25 µL, 10mM.
4-Nitrophenyl phosphate (pNPP) is a chromogenic substrate for alkaline phosphatase.1 Upon hydrolysis by alkaline phosphatase, 4-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of alkaline phosphatase activity. It has been commonly used in ELISAs.
1.Lorenz, U.Protein tyrosine phosphatase assaysCurr. Protoc. Immunol.9311.17.11-11.17.12(2011)
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