5'-deoxy Thymidine |
| Catalog No.GC10731 |
antibacterial activity
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3458-14-8
Sample solution is provided at 25 µL, 10mM.
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5'-Deoxy Thymidine has been used as a research tool for antiviral and anticancer studies. 5'-Deoxy Thymidine can be used to study the entry mechanism into human erythrocytes in comparison with other deoxythymidines. Deoxythymidine, also named as thymidine, is the DNA nucleoside T. Deoxythymidine can pair with deoxyadenosine (A) in double-stranded DNA. 5'-deoxy Thymidine is a form of thymidine which had the hydroxyl group at the 5' position replaced with hydrogen [1]. 5'-deoxy Thymidine (50 μg/mL) was effective against Bacillus subtilis and Staphylococcus aureus [1]. The MBC of 5'-deoxy Thymidine was 50 μg/mL. 5'-deoxy Thymidine has been used as an antibacterial agent in medical situations [1].
Thymidine has been phosphorylated at the hydroxyl group on carbon 5 of the ribose moiety before polymerization into DNA. 5'-Deoxy Thymidine cannot be phosphorylated and used by DNA polymerase in the synthesis of DNA. In human erythrocytes, 5'-Deoxy Thymidine was readily and nonconcentratively uptaked by metabolism, and could be partially inhibited by nucleosides or inhibitors of nucleoside transport at micromolar concentrations [2].
References:
[1] Hatano A, Nishimura M, Souta I. Impact of unnatural nucleosides on the control of microbial growth[J]. Biocontrol science, 2009, 14(2): 55-60.
[2] Domin B A, Mahony W B, Koszalka G W, et al. Membrane permeation characteristics of 5'-modified thymidine analogs[J]. Molecular pharmacology, 1992, 41(5): 950-956.
| Cas No. | 3458-14-8 | SDF | |
| Chemical Name | 5'-deoxy-thymidine | ||
| Canonical SMILES | CC1=CN(C(NC1=O)=O)[C@H]2C[C@H](O)[C@@H](C)O2 | ||
| Formula | C10H14N2O4 | M.Wt | 226.2 |
| Solubility | ≤10mg/ml in DMSO;16mg/ml in dimethyl formamide | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.4209 mL | 22.1043 mL | 44.2087 mL |
| 5 mM | 0.8842 mL | 4.4209 mL | 8.8417 mL |
| 10 mM | 0.4421 mL | 2.2104 mL | 4.4209 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)
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