Bromfenac Sodium (Synonyms: AHR 10282B) |
| Catalog No.GC13039 |
Le bromfénac sodique est un inhibiteur puissant et oralement actif de la COX, avec des CI50 de 5,56 et 7,45 nM pour la COX-1 et la COX-2, respectivement.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 91714-93-1
Sample solution is provided at 25 µL, 10mM.
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Bromfenac (sodium hydrate) is a nonsteroidal anti-inflammatory drug (NSAID), which has anti-inflammatory activity(1) Bromfenac (sodium hydrate) (1 mg/kg, i.v.) is metabolited into an unusual conjugate, bromfenac N-glucoside, in rats bile(2) Bromfenac (sodium hydrate) permeation was found to be 1.62-fold higher through ChS-CS-NPs
References:
[1]. Kirkman SK et al. Isolation and identification of bromfenac glucoside from rat bile. Drug Metab Dispos. 1998 Jul;26(7):720-3.
[2]. Abdullah TA et al. Chondroitin sulfate-chitosan nanoparticles for ocular delivery of bromfenac sodium: Improved permeation, retention, and penetration. Int J Pharm Investig. 2016 Apr-Jun;6(2):96-105.
| Cell experiment [1]: | |
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Cell lines |
Corneal epithelial cells |
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Preparation method |
The solubility of this compound in DMSO is > 14.7 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
1, 4, 12, 24 and 48 hrs |
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Applications |
Compared with Bromfenac Sodium, the cellular metabolic activity of Diclofenac and Fluorometholone markedly decreased after 12-hr exposure. However, the K+ and Cl- concentrations, pH and osmolarity were similar among different treatment groups. In additioin, Bromfenac Sodium significantly promoted cell migration, as well as restored wound gap after 48-hr exposure, compared with Diclofenac and Fluorometholone. |
| Animal experiment [2]: | |
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Animal models |
A rabbit model of ocular inflammation |
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Dosage form |
50 μL 0.09% |
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Applications |
In a rabbit model of ocular inflammation, Bromfenac Sodium almost completely inhibited lipopolysaccharide (LPS)-induced increases in fluorescein isothiocyanate (FITC)-dextran in the anterior chamber as well as the contralateral eye. In addition, Bromfenac Sodium significantly inhibited LPS-induced increases in PGE2 concentrations in the aqueous humor. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Lee JS, Kim YH, Park YM. The Toxicity of Nonsteroidal Anti-inflammatory Eye Drops against Human Corneal Epithelial Cells in Vitro. J Korean Med Sci. 2015 Dec;30(12):1856-64. [2]. Waterbury LD, Silliman D, Jolas T. Comparison of cyclooxygenase inhibitory activity and ocular anti-inflammatory effects of ketorolac tromethamine and bromfenac sodium. Curr Med Res Opin. 2006 Jun;22(6):1133-40. | |
| Cas No. | 91714-93-1 | SDF | |
| Synonymes | AHR 10282B | ||
| Chemical Name | sodium;2-[2-amino-3-(4-bromobenzoyl)phenyl]acetate | ||
| Canonical SMILES | C1=CC(=C(C(=C1)CC(=O)[O-])N)C(=O)C2=CC=C(C=C2)Br.[Na+] | ||
| Formula | C15H11BrNO3.Na | M.Wt | 356.15 |
| Solubility | ≥ 14.7mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8078 mL | 14.039 mL | 28.0781 mL |
| 5 mM | 0.5616 mL | 2.8078 mL | 5.6156 mL |
| 10 mM | 0.2808 mL | 1.4039 mL | 2.8078 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)
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