Cholera toxin (choleragen) (Synonyms: Choleragen，CT) |
| Catalog No.GC31655 |
Cholera toxin (choleragen) est un complexe protéique entérotoxigène constitué d'une seule sous-unité A (sous-unité A de la toxine cholérique (CTA)) et d'un pentamère de sous-unités B (sous-unité B de la toxine cholérique (CTB)).
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Cas No.: 9012-63-9
Sample solution is provided at 25 µL, 10mM.
Cholera toxin (choleragen) est un complexe protéique entérotoxigène constitué d'une seule sous-unité A (sous-unité A de la toxine cholérique (CTA)) et d'un pentamère de sous-unités B (sous-unité B de la toxine cholérique (CTB)). Elle pénètre dans les cellules hôtes en se liant au ganglioside GM1 présent sur la membrane plasmique, puis migre rétrogradement vers le réticulum endoplasmique via le réseau trans-Golgien [1-3].
Le traitement des cellules Jurkat avec la toxine cholérique (0,5 µg/ml ; 3 h) pendant 3 heures a provoqué une disparition quasi totale du complexe TcR à la surface des cellules [4,5]. La toxine cholérique pénètre dans les cellules épithéliales intestinales hôtes et son transport rétrograde vers le cytosol entraîne une perte massive de fluides et d'électrolytes, associée à une déshydratation sévère [6]. La toxine cholérique active l'adénylate cyclase en catalysant l'ADP-ribosylation de la sous-unité alpha de la protéine Gs, une protéine stimulatrice liant les nucléotides guanine [7].
References:
[1]. van Heyningen S. Cholera toxin. Biol Rev Camb Philos Soc. 1977 Nov;52(4):509. doi: 10.1111/j.1469-185x.1977.tb00858.x. PMID: 203347.
[2]. Wernick NL, Chinnapen DJ, et,al. Cholera toxin: an intracellular journey into the cytosol by way of the endoplasmic reticulum. Toxins (Basel). 2010 Mar;2(3):310-25. doi: 10.3390/toxins2030310. Epub 2010 Mar 5. PMID: 22069586; PMCID: PMC3153193.
[3]. Kellner A, Taylor M, et,al. A binding motif for Hsp90 in the A chains of ADP-ribosylating toxins that move from the endoplasmic reticulum to the cytosol. Cell Microbiol. 2019 Oct;21(10):e13074. doi: 10.1111/cmi.13074. Epub 2019 Jul 5. PMID: 31231933; PMCID: PMC6744307.
[4]. Sommermeyer H, Schwinzer R,et,al. Cholera toxin modulates the T cell antigen receptor/CD3 complex but not the CD2 molecule and inhibits signaling via both receptor structures in the human T cell lymphoma Jurkat. Eur J Immunol. 1989 Dec;19(12):2387-90. doi: 10.1002/eji.1830191232. PMID: 2575034.
[5]. Nunes J, Bagnasco M, et,al. Cholera toxin inhibits the increase in cytoplasmic free calcium induced via the CD2 pathway of human T-lymphocyte activation. J Cell Biochem. 1989 Apr;39(4):391-400. doi: 10.1002/jcb.240390405. PMID: 2542344.
[6]. Wands AM, Cervin J, et,al. Fucosylated Molecules Competitively Interfere with Cholera Toxin Binding to Host Cells. ACS Infect Dis. 2018 May 11;4(5):758-770. doi: 10.1021/acsinfecdis.7b00085. Epub 2018 Feb 22. PMID: 29411974; PMCID: PMC5948155.
[7]. Tsai SC, Noda M, et,al. Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain. J Biol Chem. 1988 Feb 5;263(4):1768-72. PMID: 3123477.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellules Jurkat |
Méthode de préparation | Les cellules ont été incubées pendant 3 heures avec le milieu seul ou avec la toxine cholérique (0,5 µg/ml) dans le milieu. Après lavage des cellules, l'expression de l'antigène indiqué à la surface cellulaire a été mesurée par analyse en cytométrie de flux (FCM). |
Conditions de réaction | 0,5 µg/ml ; 3 h |
Domaines d'application | Le traitement des cellules Jurkat avec la toxine cholérique pendant 3 heures a provoqué une disparition quasi totale du complexe TcR à la surface des cellules. |
Références : [1]. Nunes J, Bagnasco M, et al. Cholera toxin inhibits the increase in cytoplasmic free calcium induced via the CD2 pathway of human T-lymphocyte activation. J Cell Biochem. 1989 Apr;39(4):391-400. doi: 10.1002/jcb.240390405. PMID: 2542344. | |
| Cas No. | 9012-63-9 | SDF | |
| Synonymes | Choleragen，CT | ||
| Canonical SMILES | [Cholera toxin] | ||
| Formula | M.Wt | ||
| Solubility | When reconstituted to 0.5 ml with water, the buffer is 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5 | Storage | Store at 4°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
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