Ercalcidiol (Synonyms: Ercalcidiol, 25Hydroxyergocalciferol, 25(OH)-Vitamin D2) |
| Catalog No.GC13804 |
A metabolite of vitamin D2
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 21343-40-8
Sample solution is provided at 25 µL, 10mM.
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Ercalcidiol is a metabolite of vitamin D2 and is a VDR-like receptor [1].
Vitamin D is a group of fat-soluble secosteroids and plays an important role in enhancing intestinal absorption of calcium, iron, magnesium, phosphate and zinc. Ercalcidiol (25-hydroxy Vitamin D2) and 25-hydroxyergocalciferol (aka 25-hydroxyvitamin D2 — abbreviated 25(OH) D2) are vitamin D metabolites and Ercalcidiol is converted to 25-hydroxyergocalciferol in liver. Through measuring the level of Ercalcidiol and 25-hydroxyergocalciferol in serum can detect the person's vitamin D status [2] [3].
Using isolated perfused rat kidney model, it was demonstrated that Ercalcidiol not only converted into 24, 25-dihydroxyvitamin D2 but also converted into two other new metabolites-24, 25, 28-trihydroxyvitamin D2 and 24, 25, 26-trihydroxyvitamin D2 [4]. When tested with male weanling rat model, it was demonstrated that either a protein deficiency or a total food restriction during the early postweaning period had no effect on the synthesis and transport of Ercalcidiol and 23, 25-dihydroxyvitamin D in plama [5].
References:
[1]. Jones, G., H.K. Schnoes, and H.F. DeLuca, An in vitro study of vitamin D2 hydroxylases in the chick. J Biol Chem, 1976. 251(1): p. 24-8.
[2]. Newman, M.S., et al., A liquid chromatography/tandem mass spectrometry method for determination of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 in dried blood spots: a potential adjunct to diabetes and cardiometabolic risk screening. J Diabetes Sci Technol, 2009. 3(1): p. 156-62.
[3]. Thacher, T.D., et al., Comparison of metabolism of vitamins D2 and D3 in children with nutritional rickets. J Bone Miner Res, 2010. 25(9): p. 1988-95.
[4]. Reddy GS1, Tserng KY. 24, 25, 28-trihydroxyvitamin D2 and 24, 25, 26-trihydroxyvitamin D2: novel metabolites of vitamin D2.Biochemistry. 1990 Jan 30; 29(4):943-9.
[5]. Philbrick DJ, Hollis BW, Draper HH. Effects of protein deficiency on plasma levels of 25-hydroxyvitamin and 24, 25-dihydroxyvitamin D in the rat.
Am J Clin Nutr. 1980 Oct;33(10):2174-6.
| Cas No. | 21343-40-8 | SDF | |
| Synonymes | Ercalcidiol, 25Hydroxyergocalciferol, 25(OH)-Vitamin D2 | ||
| Chemical Name | (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(E,2R,5S)-6-hydroxy-5,6-dimethylhept-3-en-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol | ||
| Canonical SMILES | CC(C=CC(C)C(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C | ||
| Formula | C28H44O2 | M.Wt | 412.66 |
| Solubility | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml,Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml | Storage | Store at -20°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4233 mL | 12.1165 mL | 24.233 mL |
| 5 mM | 0.4847 mL | 2.4233 mL | 4.8466 mL |
| 10 mM | 0.2423 mL | 1.2117 mL | 2.4233 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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